Cover Page The handle http://hdl.handle.net/1887/37582 holds various files of this Leiden University dissertation.

 Treat to Target recommendation 5: ‘Measures of disease activity must be obtained and documented regularly as frequently as monthly for patients with high/moderate disease

Here we aimed at comparing rheumatologists’ agreement with - and their actual performance of - treat-to-target recommendations in daily clinical practice and on

Methods: Bisulfi te sequencing was performed on maternal genomic (g)DNA and fetal gDNA from chorionic villi to determine diff eren ally methylated regions in the RASSF1A gene

RHD Rhesus D gene RMD Rela ve muta on dosage RSTD Rela ve sequence tag density SMS Single molecule sequencing SNP Single nucleo de polymorphism SRY

Already at the end of the 19th century, maternal blood was considered to be a useful source of fetal gene c material for noninvasive prenatal diagnosis when the fi rst observa on

Methods: Bisulfi te sequencing was performed on maternal genomic (g)DNA and fetal gDNA from chorionic villi to determine diff eren ally methylated regions in the RASSF1A gene

Methods: For noninvasive trisomy 21 detec on, single molecule sequencing was per- formed on the Helicos pla orm using free DNA isolated from maternal plasma from 9 weeks of gesta

As a follow up on noninvasive T21 detec on using single molecule sequencing, in the present study we demonstrated successful noninvasive detec on of T18 (100% diagnos c sensi