Cover Page
The following handle holds various files of this Leiden University dissertation:
http://hdl.handle.net/1887/78473
Author: Busemann, A.
Title: Imaging of alkyne-functionalized ruthenium complexes for photoactivated
chemotherapy
Imaging of alkyne-functionalized ruthenium
complexes for photoactivated chemotherapy
PROEFSCHRIFT
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus Prof. mr. C. J. J. M. Stolker
volgens besluit van het College voor Promoties te verdedigen op dinsdag 1 oktober 2019
klokke 10:00 uur
door
Anja Busemann
Samenstelling Promotiecommissie
Promotor Dr. S. Bonnet
Prof. dr. E. Bouwman
Overige Leden Prof. dr. H. S. Overkleeft Prof. dr. M. van der Stelt
Prof. dr. S. Rau (Universität Ulm) Dr. S. Oliveira (Universiteit Utrecht)
Was mich nicht umbringt, macht mich stärker.
What does not kill me, makes me stronger.
Friedrich Nietzsche
Table of contents
CHAPTER 1
Introduction 7
CHAPTER 2
Alkyne functionalization of photoactivated ruthenium complex
[Ru(tpy)(bpy)(Hmte)](PF6)2 for protein interaction studies 29
CHAPTER 3
Ruthenium-based PACT agents:
synthesis, photochemistry, and cytotoxicity studies 49
CHAPTER 4
Visualizing the invisible:
Imaging of ruthenium-based PACT agents in fixed cancer cells 67
CHAPTER 5
Synthesis of other alkyne-functionalized ruthenium polypyridyl
complexes 89
CHAPTER 6
Summary, Discussion, and Conclusion 105
Appendix I: General experimental methods 123
Appendix II: Supporting information for Chapter 2 129
Appendix III: Supporting information for Chapter 3 137
Appendix IV: Supporting information for Chapter 4 147
Appendix V: Supporting information for Chapter 5 159
Samenvatting 165
Zusammenfassung 169
List of Publications 175
Curriculum Vitae 177
