University of Groningen
Bridging the gap
Spiekman, Maroesjka
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Publication date:
2018
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Spiekman, M. (2018). Bridging the gap: Adipose tissue-based therapy for dermal scarring. Rijksuniversiteit
Groningen.
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Bridging the gap
Adipose tissue-based therapy for dermal scarring
Proefschrift
ter verkrijging van de graad van doctor aan de
Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. E. Sterken
en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op
woensdag 20 juni 2018 om 12.45 uur
door
Maroesjka Spiekman
geboren op 11 augustus 1990
te Groningen
For publication of this thesis, financial support from the Graduate School of Medical Sciences of the University of Groningen / University Medical Center Groningen, Human Med AG, Schwerin, Germany and BlooMEDical Benelux BV is gratefully acknowledged.
The research in this thesis was supported by the University Medical Center Groningen, University of Groningen and by grants from the Junior Scientific Masterclass, The Ubbo Emmius Fund, Jan Kornelis de Cock Foundation and Foundation ‘De Drie Lichten’.
Spiekman, Maroesjka
Bridging the gap: Adipose tissue-based therapy for dermal scarring ISBN: 978-94-034-0664-0
ISBN: 978-94-034-0663-3 / elektronische versie Cover design: H.W. Spiekman
Lay-out: Rozemarijn Klein Heerenbrink, PersoonlijkProefschrift.nl Printed by: Ipskamp Printing B.V.
© Maroesjka Spiekman
All rights reserved. No part of this publication may be reproduced, stored on a retrieval system, or transmitted in any form or by any means, without permission of the author.
Promotores
Prof. dr. M.C. Harmsen Prof. dr. B. van der Lei
Copromotor
Dr. G. Krenning
Beoordelingscommissie
Prof. dr. M.F. Jonkman Prof. dr. R.A. Bank Prof. dr. P.P.M. Zuijlen
Paranimfen
Marloes A.M. Peters Byambasuren Vanchin
TABLE OF CONTENTS
CHAPTER 1 Introduction and outline 9
CHAPTER 2 The power of fat and its adipose-derived stromal cells: emerging concepts for fibrotic scar treatment
31
CHAPTER 3 Autologous lipofilling improves clinical outcome in patients with symptomatic dermal scars through induction of a pro-regenerative immune response
63
CHAPTER 4 A comparison of intraoperative procedures for isolation of clinical grade stromal vascular fraction for regenerative purposes: a systematic review
93
CHAPTER 5 The role of stromal vascular fraction in scar prevention: a model for a well-designed prospective double blind randomized placebo-controlled clinical trial
131
CHAPTER 6 Platelet-Rich Plasma (PRP) influences expansion and paracrine function of adipose-derived stromal cells (ADSC) in a dose-dependent fashion
147
CHAPTER 7 Adipose tissue-derived stromal cells inhibit TGF-β1-induced differentiation of human dermal fibroblasts and keloid scar-derived fibroblasts in a paracrine fashion
169
CHAPTER 8 MicroRNA-15b is decreased during cardiac fibrosis and inhibits cardiac fibroblast activation through targeting of the small GTPase intermediates Growth Factor Receptor-Bound 2 (Grb2) and Son-of- Sevenless homologue (SOS) 1 and SOS2
189
CHAPTER 9 Research summary and general discussion
215
CHAPTER 10 Nederlandse samenvatting
243
APPENDICES Abbreviations Curriculum vitae Grants & Awards Presentations & Lectures List of publications Dankwoord
