Cover Page
The handle
http://hdl.handle.net/1887/138678
holds various files of this Leiden
University dissertation.
Author:
Goldhaber Pasillas, G.D.
Title:
The early stress response of jasmonic acid in cell suspension cultures of
Catharanthus roseus
The early stress response of jasmonic acid in cell
suspension cultures of Catharanthus roseus
Goldhaber Pasillas, Guitele Dalia
The early stress response of jasmonic acid in cell suspension cultures of Catharanthus roseus PhD thesis Leiden University, The Netherlands
Front cover: designed by Marco Danton Herrejón Romero and Guitele Dalia Goldhaber Pasillas Thesis lay-out by Guitele Dalia Goldhaber Pasillas
Printing and binding: PrintSupport4U ISBN: 978-94-92597-59-5
The early stress response of jasmonic acid in cell
suspension cultures of Catharanthus roseus
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties te verdedigen op woensdag 16 december 2020
klokke 10:00 uur
door
Guitele Dalia Goldhaber Pasillas geboren te Mexico Stad, Mexico
Promotor
Prof. dr. Robert Verpoorte
Copromotor
Dr. Annelies Elisabeth Schulte
Promotiecommissie Prof. dr. Gilles van Wezel Prof. dr. Annemarie H. Meijer Prof. dr. Remko Offringa
Dr. Teris van Beek (Wageningen University & Research, Wageningen, The Netherlands) Dr. María del Pilar López Gresa (Universitat Politècnica de València, Valencia, Spain)
This work was supported by Consejo Nacional de Ciencia y Tecnología (CONACYT) to Guitele Dalia Goldhaber Pasillas
Most people say that is the intellect which makes a great scientist. They are wrong, it is character. -Albert Einstein-
C
ONTENTS
Chapter 1 General introduction 1 Chapter 2 Fatty acid profiles of cell suspension cultures and intact organs of
Catharanthus roseus using a gas chromatography-mass spectrometry targeted approach
39
Chapter 3 Effect of jasmonic acid on fatty acids of cell suspension cultures of
Catharanthus roseus 67
Chapter 4 Terpenoid indole alkaloid profiles of cell suspension cultures of
Catharanthus roseus 85
Chapter 5 Effects of dinor-12-oxo-phytodienoic acid feeding and study of the early steps of jasmonic acid biosynthesis in cell suspension cultures of Catharanthus roseus
113
Chapter 6 Metabolism of exogenous jasmonic acid in cell suspension cultures of
Catharanthus roseus 143
Chapter 7 General discussion & perspectives 165
Summary in English 175
Summary in Dutch 179
Acknowledgements 183
List of publications 184
