Cover Page
The handle http://hdl.handle.net/1887/80758 holds various files of this Leiden University
dissertation.
Author: Halaidych, O.V.
Title: Towards functional analysis of cerebrovascular cell types derived from human
induced pluripotent stem cells
Towards Functional Analysis of
Cerebrovascular Cell Types Derived from
Human Induced Pluripotent Stem Cells
Colophon
Towards Functional Analysis of Cerebrovascular Cell Types Derived from Human Induced Pluripotent Stem Cells
PhD thesis
This thesis was prepared at the department of Anatomy and Embryology of the Leiden University Medical Center, Leiden, the Netherlands
Copyright © 2019 Oleh V. Halaidych
All rights reserved. No part of this book may be reproduced or transmitted, in any form or by any means, without permission in writing of the author. The copyright of the articles that have been published has been transferred to the respective journals.
The research described in this thesis was supported by the European Research Council and a grant from Møller Foundation
ISBN: 978-617-7450-15-2
Cover: coculture of endothelial (blue) and smooth muscle cells (green). Image is courtesy of Amy Cochrane
Design: Oleh Halaidych
Publisher: ”Yurait” Kharkiv, Ukraine, 2019.
Towards Functional Analysis of
Cerebrovascular Cell Types Derived from
Human Induced Pluripotent Stem Cells
Proefschrift ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties te verdedigen op
woensdag 20 november 2019 klokke 13:45 uur
door
Oleh Viktorovych Halaidych
Promotor: Prof. dr. C. L. Mummery Co-promotor: Dr. V. V. Orlova Promotiecommissie:
Prof. dr. A.J. van Zonneveld Dr. F.P.G. Lebrin
Contents
Chapter 1 Introduction and scope of this thesis 7 Chapter 2 Inflammatory responses and barrier function of 49
endothelial cells derived from hiPSC.
Chapter 3 Microfluidic assay for the assessment of inflammatory 89 responses of human induced pluripotent stem
cell-derived endothelial cells (hiPSC-ECs).
Chapter 4 Quantifying Ca2+ signaling and contraction in vascular 105
pericytes and smooth muscle cells.
Chapter 5 Quantitative analysis of intracellular Ca2+ release and 125
contraction in hiPSC-derived vascular smooth muscle cells.
