University of Groningen
Terpenoid cell factory
Abdallah, Ingy Ibrahim Ahmed Fouad
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Publication date: 2018
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Abdallah, I. I. A. F. (2018). Terpenoid cell factory. Rijksuniversiteit Groningen.
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STELLINGEN
Behorende bij het proefschrift
Terpenoid Cell Factory
van Ingy I. Abdallah1. Over expression of the innate MEP pathway of Bacillus subtilis developed it into a promising cell factory for production of valuable terpenoids (This thesis).
2. Terpene synthases possess an intriguing catalytic mechanism and unraveling their structure-function relations is a challenging but exciting task (This thesis).
3. Computational modeling is a very useful tool for enzyme engineering; however it should go hand in hand with experimental results (This thesis).
4. Mutability landscape guided engineering provided insight into the mechanism of amorpha-4,11-diene synthase and opened the path for possibly improving production of the antimalarial artemisinin (This thesis).
5. A single amino acid substitution can have a great impact on the catalytic activity and product profile of amorpha-4,11-diene synthase (This thesis).
6. Metabolic engineering experiments are both frustrating and rewarding. 7. Cloning would have been an enjoyable task, if it can be physically
experienced as assembling LEGO.
8. I believe that the statement by Anatole France “To accomplish great things, we must not only act, but also dream; not only plan, but also believe” is the essence of research.
9. Limits exist only in the mind so don’t be afraid to push the boundaries and discover novel landscapes.
