Gas chromatography mass spectrometry: key technology in metabolomics
Koek, Maud Marijtje
Citation
Koek, M. M. (2009, November 10). Gas chromatography mass spectrometry: key technology in metabolomics. Retrieved from https://hdl.handle.net/1887/14328
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
Downloaded from: https://hdl.handle.net/1887/14328
Note: To cite this publication please use the final published version (if applicable).
Gas chromatography mass spectrometry:
key technology in metabolomics
Proefschrift ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van Rector Magnificus prof.mr. P.F. van der Heijden, volgens besluit van het College voor Promoties
te verdedigen op 10 november 2009 klokke 15:00 uur
door
Maud Marijtje Koek geboren te Leiderdorp
in 1974
Promotiecommissie
Promotor: Prof. dr. Th. Hankemeier Overige leden: Dr. ir. L. Coulier
Prof. dr. M. Danhof Prof. dr. J. van der Greef Prof. dr. M.D. Ferrari
Prof. dr. ir. P.J. Schoenmakers Dr. ir. M.J. van der Werf
ISBN: 978-90-74538-69-5
Publication of this thesis was financially supported by TNO Quality of life, Zeist, The Netherlands
Printed by Wöhrmann print service, Zutphen, The Netherlands
Contents
1 General introduction and scope ... 5
2 Quantitative metabolomics based on gas chromatography-mass spectrometry: status and perspectives ... 15
3 Microbial metabolomics with gas chromatography-mass spectrometry ... 49
4 Towards quantitative metabolomics with silylation-based gas chromatography-mass spectrometry: key issues and recommendations ... 73
5 Higher mass loadability in GC×GC–MS: improved analytical performance for metabolomics analysis ... 105
6 Automated non-target processing in GC×GC-MS metabolomics analysis: applicability for biomedical studies ... 129
7 Metabolic profiling of ultra small sample volumes with GC-MS: from microlitres to nanolitres samples ... 157
8 Conclusions and perspectives ... 175
Summary ... 181
Samenvatting ... 183
Nawoord ... 185
Curriculum vitae ... 187
List of publications ... 189
