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Characterization of B cell responses in relation to organ transplantation Heidt, S.

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Heidt, S.

Citation

Heidt, S. (2010, March 3). Characterization of B cell responses in relation to organ transplantation. Retrieved from https://hdl.handle.net/1887/15051

Version: Corrected Publisher’s Version

License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden

Downloaded from: https://hdl.handle.net/1887/15051

Note: To cite this publication please use the final published version (if applicable).

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Abbreviations

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Abbreviations

159 ABBREVIATIONS

3H-TdR 3H-tritiated thymidine 3P Third party

aa Amino acid

ADCC Antibody dependent cytotoxicity APCs Antigen presenting cells

ATG Anti-thymocyte globulin BCPF B cell precursor frequency BCR B cell receptor

B-LCL B lymphoblastoid cell line

BOS Bronchiolitis obliterans syndrome

bp Base pairs

BSA Bovine serum albumin

CAN Chronic allograft nephropathy CBA Cytometric bead array

CDC Complement dependent cytotoxicity CFSE Carboxyfluorescein succinimidyl ester CTL Cytotoxic T lymphocyte

CTLp Cytotoxic T lymphocyte precursor

CpG DNA Cytosine-guanine dinucleotide oligodeoxynucleotide CPM Counts per minute

CyP Cyclophilin

DAR Direct allorecognition DC Dendritic cell DMSO Dimethyl sulfoxide DNA Deoxyribonucleic acid

EDTA Ethylenediaminetetraacetic acid ELISA Enzyme-linked immunosorbent assay ELISPOT Enzyme-linked immunosorbent spot Fab Fragment antigen-binding

Fc Fragment crystallizable FcR Fc receptor

FcRn Neonatal Fc receptor FCS Fetal calf serum

FCCS Flow cytometry cytokine secretion assay

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160

FCM Flow cytometric analysis FDC Follicular dendritic cell FKBP FK506-binding protein Foxp3 Forkhead box P3

HLA Human leukocyte antigen

HPLC High performance liquid chromatography IAR Indirect allorecognition

ICOS Inducible costimulator IFN-γ Interferon-γ

IFTA Interstitial fibrosis and tubular atrophy

Ig Immunoglobulin

IL Interleukin i.v. Intravenous

IMDM Iscove’s modified Dulbecco’s medium IMPDH Inosine-5’-monophosphate dehydrogenase IVIg Intravenous immunoglobulin

LDA Limiting dilution analysis LDH Lactate dehydrogenase mAb Monoclonal antibody MFI Mean fluorescence intensity MHC Major histocompatibility complex MLC Mixed lymphocyte culture MMF Mycophenolate mofetil MPA Mycophenolic acid mRNA Messenger RNA

mTOR Mamalian target of rapamycin NFAT Nuclear factor of activated T cells NF-κB Nuclear factor-κB

NK cell Natural killer cell

PBMC Peripheral blood mononuclear cells PBS Phosphate buffered saline

PCR Polymerase chain reaction PEG Polyethylene glycol PHA Phytohaemagglutinin

PMSF Phenylmethylsulphonyl fluoride PWM Pokeweed mitogen

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Abbreviations

161 PI Propidium Iodide

PRA Panel reactive antibodies PTC Peritubular capillary

Q-PCR Quantitative polymerase chain reaction

RhD Rhesus D

RIA Radioimmunoassay RNA Ribonucleic acid

RPMI Roswell Park Memorial Institute medium

RT Room temperature

RT-AMV Reverse transcriptase-avian myeloblastosis virus SAL Single antigen line

TCL T cell line TCC T cell clone TCR T cell receptor

Th T helper

TLR Toll like receptor UCB Umbilical cord blood

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