Cover Page
The handle http://hdl.handle.net/1887/80689 holds various files of this Leiden University
dissertation.
Author: Sun, Z.
Title: A semisynthetic peptide-metalloporphyrin responsive matrix for artificial
photosynthesis
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Index
Contents
Abbreviations ... 5Chapter 1
A general introduction ... 71.1 Moving from natural photosynthesis to artificial photosynthesis ... 7
1.2 The natural and artificial light-harvesting antenna ... 9
1.3 Non-adiabatic Conversion by Adiabatic Passage (NCAP) processes ... 12
1.4 Constitution of an artificial light-harvesting antenna complex ... 13
1.4.1 The synthesis of tCsmA ... 13
1.4.2 The constitution of the tCsmA-ZnPP complex ... 15
1.5 Structure determination ... 16
1.6 Single particle analysis for helical structure ... 18
1.7 Scope of this thesis ... 19
1.8 References ... 20
Chapter 2
Self-assembly of a semi-synthetic zinc-protoporphyrin IX peptide complex derived from the natural CsmA light harvesting antenna ... 232.0 Abstract ... 23
2.1 Introduction ... 24
2.2 Experimental section ... 25
2
2.2.2 Equipments ... 26
2.2.3 Methods ... 26
2.3 Results and discussion ... 28
2.3.1 Constitution and characterization ... 28
2.3.2 Structural information ... 32 2.3.3 Photostability ... 35 2.3.4 Exciton-energy transfer ... 37 2.4 Conclusions ... 39 2.5 References ... 40 2.6 Appendix ... 43
Chapter 3
Contrasting modes of modulated self-assembly for CsmA homologues coordinated to ZnPP ... 49 3.0 Abstract ... 49 3.1 Introduction ... 50 3.2 Experimental section ... 51 3.2.1 Materials ... 51 3.2.2 Equipment ... 51 3.2.3 Methods ... 513.3 Results and discussion ... 52
3.3.1 Conditions for self-assembly of the tCsmA-ZnPP complex ... 52
3.3.2 The helical structure of the self-assembly... 56
3
3.4 Conclusion ... 62
3.5 References ... 63
Chapter 4
Cryo-EM structure of the self-assembly of tCsmA and ZnPP ... 654.0 Abstract ... 65
4.1 Introduction ... 66
4.2 Experimental section ... 67
4.3 Results and discussion ... 68
4.3.1 Cryo-EM images processing of the helical self-assembly ... 68
4.3.2 Cryo-EM structure of the helical self-assembly... 71
4.4 Conclusion ... 73
4.5 References ... 75
4.6 Appendix ... 76
Chapter 5
Purification of recombinant variants of tCsmA ... 775.0 Abstract ... 77
5.1 Introduction ... 78
5.2 Experimental section ... 78
5.2.1 Materials ... 78
5.2.2 Methods ... 80
5.3 Results and discussion ... 83
4 5.3.2 Protein purification ... 85 5.4 Conclusions ... 88 5.5 References ... 90 5.6 Appendix ... 91
Chapter 6
General discussion and outlook ... 936.1 Structure assessment ... 93
6.2 Constitution ... 94
6.3 Photochemical relevance ... 95
6.4 Future experiments ... 96
6.4.1 Functional exploration... 96
6.4.2 Atomic structure determination ... 97
6.5 References ... 99
Abbreviations
5
Abbreviations
3DEM
3D Electron Microscopy
BCA
Bicinchoninic acid
BChl
Bacteriochlorophyll
CD
Circular dichroism
CMC
Critical micelle concentration
Cryo-EM
Cryogenic electron microscopy
CsmA
Chlorosome protein A
CTF
Contrast transfer function
DMSO
Dimethyl sulfoxide
E.coli
Escherichia coli
ET
Electron tomography
FMO
Fenna matthews olson
GuHCl
Guanidine hydrochloride
HG
Hexyl β-D-glucopyranoside
ICP-OES
Inductive coupled plasma optical emission spectrometry
IPTG
Isopropyl β- d-1-thiogalactopyranoside
KPi
Potassium phosphate
LD
Linear dichroism
MBP
Maltose-binding protein
MV
Methyl viologen
NCAP
Non-adiabatic conversion by adiabatic passage
NMR
Nuclear magnetic resonance
OG
Octyl β-D-glucopyranoside
RP-HPLC
Reverse phase high-performance liquid chromatography
Abbreviations
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