Supplementary Material Table of contents

(1)

1. ABI capillary electrophoresis results ... 2 2. GX results ... 18 3. Experion results ... 44

(2)

Results

1. ABI capillary electrophoresis results

Gel electrophoresis UGT1A1 gene – TA-repeat

Gel lay out: Ladders (Gene ruler and Fast ruler) and Saliva samples + Negative control Gel of S1-8

Gel of S9-24 with positive and negative control

Gel of S25-40 with positive and negative control

(3)

252.23 250.15

0 300 600 900

A01_1_LBO.fsa 11 180-280

252.15 250.15

0 7000 14000 21000

202.5 217.5 232.5 247.5 262.5 277.5

C01_3_LBO.fsa 33 180-280

250.17

0 9000 18000 27000

202.5 217.5 232.5 247.5 262.5 277.5

F01_6_LBO.fsa 66 180-280

250.19

0 8000 16000 24000

202.5 217.5 232.5 247.5 262.5 277.5

(4)

Sample File

Sample File Sample NameSample Name MarkerMarker OSOS SPUSPU GQGQ

GeneMapper 4.1

MYOZ2_Pieter_11dec

B01_2_LBO.fsa 22 180-280

250.17

0 7000 14000 21000

202.5 217.5 232.5 247.5 262.5 277.5

E01_5_LBO.fsa 55 180-280

250.13

0 4000 8000 12000

202.5 217.5 232.5 247.5 262.5 277.5

G01_7_LBO.fsa 77 180-280

250.13

0 5000 10000 15000

202.5 217.5 232.5 247.5 262.5 277.5

H01_8_LBO.fsa 88 180-280

5000 10000 15000

202.5 217.5 232.5 247.5 262.5 277.5

(5)

250.24

0 1200 2400 3600

G03_S31_LBO.fsa S31S31 180-280

250.22

0 1100 2200 3300

200 220 240 260 280 300

G04_S39_LBO.fsa S39S39 180-280

250.22

0 4000 8000 12000

200 220 240 260 280 300

(6)

Sample File

GeneMapper 4.1

Markers_prathima

H01_S16_LBO.fsa S16S16 180-280

250.04

0 1500 3000 4500

200 220 240 260 280 300

H04_S40_LBO.fsa S40S40 180-280

250.20

0 170 340 510

200 220 240 260 280 300

(7)

0 40 80 120

A04_S33_LBO.fsa S33S33 180-280

252.31 250.26

0 500 1000 1500

200 220 240 260 280 300

A01_S9_LBO.fsa S9S9 180-280

252.22 250.19

0 3000 6000 9000

200 220 240 260 280 300

(8)

Sample File

GeneMapper 4.1

Markers_prathima

B02_S18_LBO.fsa S18S18 180-280

249.95

0 8000 16000 24000

200 220 240 260 280 300

B04_S34_LBO.fsa S34S34 180-280

252.24 250.28

0 400 800 1200

200 220 240 260 280 300

(9)

252.29 250.24

0 400 800 1200

D03_S28_LBO.fsa S28S28 180-280

252.18 250.22

0 4000 8000 12000

200 220 240 260 280 300

(10)

Sample File

GeneMapper 4.1

Markers_prathima

E02_S21_LBO.fsa S21S21 180-280

252.17 250.22

0 400 800 1200

200 220 240 260 280 300

E03_S29_LBO.fsa S29S29 180-280

252.12 250.15

0 300 600 900

200 220 240 260 280 300

(11)

252.21 250.15

0 600 1200 1800

H02_S24_LBO.fsa S24S24 180-280

252.12 250.11

0 800 1600 2400

200 220 240 260 280 300

(12)

Sample File

GeneMapper 4.1

Markers_prathima

H03_S32_LBO.fsa S32S32 180-280

252.20 250.17

0 800 1600 2400

200 220 240 260 280 300

A02_S17_LBO.fsa S17S17 180-280

250.19

0 3000 6000 9000

200 220 240 260 280 300

(13)

250.24

0 4000 8000 12000

B03_S26_LBO.fsa S26S26 180-280

250.22

0 800 1600 2400

200 220 240 260 280 300

C01_S11_LBO.fsa S11S11 180-280

250.15

0 1800 3600 5400

200 220 240 260 280 300

(14)

Sample File

GeneMapper 4.1

Markers_prathima

C03_S27_LBO.fsa S27S27 180-280

252.29

0 800 1600 2400

200 220 240 260 280 300

C04_S35_LBO.fsa S35S35 180-280

252.24

0 800 1600 2400

200 220 240 260 280 300

D01_S12_LBO.fsa S12S12 180-280

(15)

250.09

0 700 1400 2100

E04_S37_LBO.fsa S37S37 180-280

250.27

0 800 1600 2400

200 220 240 260 280 300

F01_S14_LBO.fsa S14S14 180-280

250.11

0 3000 6000 9000

200 220 240 260 280 300

(16)

Sample File

GeneMapper 4.1

Markers_prathima

F02_S22_LBO.fsa S22S22 180-280

250.24

0 1900 3800 5700

200 220 240 260 280 300

F03_S30_LBO.fsa S30S30 180-280

250.22

0 1600 3200 4800

200 220 240 260 280 300

G01_S15_LBO.fsa S15S15 180-280

(17)

0 7 14 21

B05_neg_control_LBO.fsa neg_controlneg_control 180-280 -4.0 -4.0

0 12 24 36

200 220 240 260 280 300

A05_pos_control_LBO.fsa pos_controlpos_control 180-280

252.29 248.28

0 2300 4600 6900

200 220 240 260 280 300

(18)

2. GX results

See next pages for the results retrieved from the LabChip GX.

(19)

L L L L 1 2 3 4 5 6

-

0 -⁰

-

50 -⁵⁰

-

100 -¹⁰⁰

-

150 -¹⁵⁰

-

200 -²⁰⁰

-

300 -³⁰⁰

-

400 -⁴⁰⁰

-

500 -⁵⁰⁰

-

700 -⁷⁰⁰

-

1100 -¹¹⁰⁰

-

1900 -¹⁹⁰⁰

-

2900 -²⁹⁰⁰

-

4900- -⁴⁹⁰⁰

7000 -⁷⁰⁰⁰

(20)

Page 2 Collection 1

7 8 1A 2A 3A 4A 5A 6A 7A 8A

-

100 -¹⁰⁰

-

150 -¹⁵⁰

-

200 -²⁰⁰

-

300 -³⁰⁰

-

400 -⁴⁰⁰

-

500 -⁵⁰⁰

-

700 -⁷⁰⁰

-

1100 -¹¹⁰⁰

-

1900 -¹⁹⁰⁰

-

2900 -²⁹⁰⁰

-

4900- -⁴⁹⁰⁰

7000 -⁷⁰⁰⁰

(21)

1 2 3 4 5 6 7 8 1 2

-

0 -⁰

-

50 -⁵⁰

-

100 -¹⁰⁰

-

150 -¹⁵⁰

-

200 -²⁰⁰

-

300 -³⁰⁰

-

400 -⁴⁰⁰

-

500 -⁵⁰⁰

-

700 -⁷⁰⁰

-

1100 -¹¹⁰⁰

-

1900 -¹⁹⁰⁰

-

2900 -²⁹⁰⁰

-

4900- -⁴⁹⁰⁰

7000 -⁷⁰⁰⁰

(22)

Page 4 Collection 1

3A 4A 5A 6A 7A 8A blank blank blank blank

-

100 -¹⁰⁰

-

150 -¹⁵⁰

-

200 -²⁰⁰

-

300 -³⁰⁰

-

400 -⁴⁰⁰

-

500 -⁵⁰⁰

-

700 -⁷⁰⁰

-

1100 -¹¹⁰⁰

-

1900 -¹⁹⁰⁰

-

2900 -²⁹⁰⁰

-

4900- -⁴⁹⁰⁰

7000 -⁷⁰⁰⁰

(23)

Ladder01 Ladder1 300 0.250 1.263

Ladder01 Ladder1 400 0.250 0.947

Ladder01 Ladder1 500 0.250 0.758

Ladder01 Ladder1 700 0.250 0.541

Ladder01 Ladder1 1100 0.250 0.344

Ladder01 Ladder1 1900 0.250 0.199

Ladder01 Ladder1 2900 0.250 0.131

Ladder01 Ladder1 4900 0.250 0.077

Ladder01 Ladder1 7000 0.500 0.108

Ladder02 Ladder2 1.000

Ladder02 Ladder2

Ladder02 Ladder2 50 0.250 7.576

Ladder02 Ladder2 100 0.250 3.788

Ladder02 Ladder2 150 0.250 2.525

Ladder02 Ladder2 200 0.250 1.894

Ladder02 Ladder2 300 0.250 1.263

Ladder02 Ladder2 400 0.250 0.947

Ladder02 Ladder2 500 0.250 0.758

Ladder02 Ladder2 700 0.250 0.541

Ladder02 Ladder2 1100 0.250 0.344

Ladder02 Ladder2 1900 0.250 0.199

Ladder02 Ladder2 2900 0.250 0.131

Ladder02 Ladder2 4900 0.250 0.077

Ladder02 Ladder2 7000 0.500 0.108

Ladder03 Ladder3 0

Ladder03 Ladder3

Ladder03 Ladder3 50 0.250 7.576

Ladder03 Ladder3 76

Ladder03 Ladder3 100 0.250 3.788

Ladder03 Ladder3 150 0.250 2.525

Ladder03 Ladder3 200 0.250 1.894

Ladder03 Ladder3 300 0.250 1.263

Ladder03 Ladder3 400 0.250 0.947

Ladder03 Ladder3 500 0.250 0.758

Ladder03 Ladder3 700 0.250 0.541

Ladder03 Ladder3 1100 0.250 0.344

Ladder03 Ladder3 1900 0.250 0.199

Ladder03 Ladder3 2900 0.250 0.131

Ladder03 Ladder3 3753

Ladder03 Ladder3 4900 0.250 0.077

Ladder03 Ladder3 5761

Ladder03 Ladder3 7000 0.500 0.108

Ladder03 Ladder3 0

Ladder04 Ladder4

Ladder04 Ladder4 50 0.250 7.576

Ladder04 Ladder4 100 0.250 3.788

Ladder04 Ladder4 150 0.250 2.525

Ladder04 Ladder4 200 0.250 1.894

Ladder04 Ladder4 300 0.250 1.263

Ladder04 Ladder4 400 0.250 0.947

(24)

Page 6 Collection 1

Well Label Sample Name

Size [BP] Conc.

(ng/ul)

Molarity (nmol/l)

Ladder04 Ladder4 500 0.250 0.758

Ladder04 Ladder4 700 0.250 0.541

Ladder04 Ladder4 1100 0.250 0.344

Ladder04 Ladder4 1900 0.250 0.199

Ladder04 Ladder4 2900 0.250 0.131

Ladder04 Ladder4 4900 0.250 0.077

Ladder04 Ladder4 7000 0.500 0.108

I01 1 1.000

I01 1

I01 1 7 0.265 57.314

I01 1 13 0.050 5.984

I01 1 16 0.043 4.067

I01 1 29 0.101 5.281

I01 1 78 0.110 2.156

I01 1 6377 0.545 0.129

I01 1 7000 0.500 0.108

I01 1

J01 2 1.000

J01 2

J01 2 7 0.187 41.620

J01 2 13 0.065 7.879

J01 2 29 0.073 3.807

J01 2 78 0.076 1.484

J01 2 1575 0.013 0.012

J01 2 3960 0.100 0.038

J01 2 6518 0.972 0.226

J01 2 7000 0.500 0.108

J01 2

K01 3 1.000

K01 3

K01 3 0 0.010 56.149

K01 3 7 0.084 18.841

K01 3 12 0.016 2.038

K01 3 15 0.019 2.017

K01 3 29 0.037 1.917

K01 3 77 0.036 0.698

K01 3 4073 0.007 0.002

K01 3 7000 0.500 0.108

K01 3

(25)

M01 5

N01 6 1.000

N01 6

N01 6 6 0.108 26.181

N01 6 12 0.022 2.846

N01 6 16 0.021 2.003

N01 6 28 0.044 2.363

N01 6 75 0.047 0.943

N01 6 3484 0.492 0.214

N01 6 4845 0.026 0.008

N01 6 6212 0.269 0.066

N01 6 7000 0.500 0.108

N01 6

O01 7 1.000

O01 7

O01 7 6 0.106 27.000

O01 7 13 0.019 2.220

O01 7 28 0.044 2.380

O01 7 75 0.048 0.965

O01 7 1517 0.009 0.009

O01 7 3707 0.050 0.020

O01 7 6181 0.531 0.130

O01 7 7000 0.500 0.108

O01 7

P01 8 1.000

P01 8

P01 8 6 0.149 36.324

P01 8 12 0.030 3.968

P01 8 15 0.018 1.886

P01 8 28 0.051 2.756

P01 8 76 0.045 0.901

P01 8 3986 0.037 0.014

P01 8 7000 0.500 0.108

P01 8

A02 1A 1.000

A02 1A

A02 1A 15 0.041 3.990

A02 1A 28 0.016 0.890

A02 1A 36 0.026 1.111

A02 1A 142 0.010 0.106

A02 1A 3345 0.051 0.023

A02 1A 3698 0.021 0.008

A02 1A 4648 0.497 0.162

A02 1A 7000 0.500 0.108

(26)

Page 8 Collection 1

(ng/ul)

A02 1A

B02 2A 1.000

B02 2A

B02 2A 15 0.039 3.937

B02 2A 28 0.014 0.765

B02 2A 36 0.022 0.949

B02 2A 142 0.008 0.081

B02 2A 3421 0.035 0.016

B02 2A 4727 0.449 0.144

B02 2A 7000 0.500 0.108

B02 2A

C02 3A 1.000

C02 3A

C02 3A 16 0.030 2.874

C02 3A 28 0.015 0.789

C02 3A 35 0.008 0.357

C02 3A 3439 0.015 0.007

C02 3A 4803 0.329 0.104

C02 3A 6043 0.173 0.043

C02 3A 7000 0.500 0.108

C02 3A

D02 4A 1.000

D02 4A

D02 4A 16 0.037 3.523

D02 4A 36 0.022 0.919

D02 4A 143 0.012 0.124

D02 4A 3377 0.027 0.012

D02 4A 4799 0.440 0.139

D02 4A 7000 0.500 0.108

(27)

F02 6A 3443 0.224 0.098

F02 6A 3992 0.046 0.017

F02 6A 4597 0.123 0.041

F02 6A 4789 0.129 0.041

F02 6A 5932 0.091 0.023

F02 6A 7000 0.500 0.108

F02 6A

G02 7A 1.000

G02 7A

G02 7A 15 0.095 9.713

G02 7A 27 0.043 2.418

G02 7A 34 0.029 1.319

G02 7A 35 0.031 1.318

G02 7A 243 0.047 0.294

G02 7A 1807 0.019 0.016

G02 7A 2558 0.062 0.037

G02 7A 2607 0.102 0.059

G02 7A 3118 1.079 0.524

G02 7A 5085 1.908 0.568

G02 7A 7000 0.500 0.108

G02 7A

H02 8A 1.000

H02 8A

H02 8A 16 0.034 3.136

H02 8A 29 0.018 0.949

H02 8A 37 0.017 0.690

H02 8A 77 0.025 0.493

H02 8A 90 0.013 0.217

H02 8A 293 0.006 0.031

H02 8A 3388 0.031 0.014

H02 8A 3827 0.035 0.014

H02 8A 4679 0.263 0.085

H02 8A 5967 0.148 0.038

H02 8A 7000 0.500 0.108

H02 8A

I02 1

I02 1 1.000

I02 1

(28)

Page 10 Collection 1

(ng/ul)

I02 1

I02 1 6 0.047 12.402

I02 1 7 0.045 9.153

I02 1 13 0.013 1.490

I02 1 17 0.011 0.924

I02 1 29 0.035 1.818

I02 1 81 0.036 0.684

I02 1 850 0.012 0.021

I02 1 885 0.014 0.024

I02 1 918 0.019 0.031

I02 1 961 0.005 0.008

I02 1 1020 0.006 0.010

I02 1 1079 0.009 0.012

I02 1 7000 0.500 0.108

I02 1

J02 2

J02 2 1.000

J02 2

J02 2 8 0.052 10.403

J02 2 17 0.013 1.123

J02 2 29 0.036 1.886

J02 2 30 0.016 0.825

J02 2 66 0.004 0.099

J02 2 81 0.027 0.511

J02 2 125 0.014 0.170

J02 2 143 0.017 0.179

J02 2 332 0.006 0.028

J02 2 765 0.008 0.016

J02 2 1595 0.004 0.003

J02 2 1661 0.008 0.008

J02 2 1841 0.010 0.009

J02 2 1942 0.004 0.003

J02 2 2338 0.004 0.003

J02 2 4303 0.035 0.012

J02 2 7000 0.500 0.108

J02 2

(29)

K02 3 430 0.007 0.024

K02 3 483 0.008 0.025

K02 3 494 0.006 0.018

K02 3 792 0.009 0.017

K02 3 875 0.017 0.030

K02 3 910 0.014 0.024

K02 3 926 0.023 0.038

K02 3 990 0.007 0.011

K02 3 1125 0.004 0.006

K02 3 1499 0.005 0.005

K02 3 1553 0.008 0.008

K02 3 1957 0.005 0.004

K02 3 2814 0.004 0.002

K02 3 3850 0.008 0.003

K02 3 7000 0.500 0.108

K02 3

L02 4 1.000

L02 4

L02 4 7 0.143 31.704

L02 4 13 0.025 2.914

L02 4 16 0.015 1.384

L02 4 29 0.060 3.141

L02 4 80 0.053 0.999

L02 4 135 0.015 0.167

L02 4 237 0.006 0.040

L02 4 410 0.010 0.037

L02 4 1365 0.013 0.015

L02 4 6725 0.410 0.092

L02 4 7000 0.500 0.108

L02 4

M02 5

M02 5 1.000

M02 5

M02 5 5 0.103 28.539

M02 5 11 0.024 3.272

M02 5 15 0.028 2.920

M02 5 27 0.044 2.464

M02 5 62 0.012 0.296

M02 5 77 0.047 0.935

M02 5 265 0.012 0.067

M02 5 5317 0.009 0.003

(30)

(ng/ul)

M02 5 7000 0.500 0.108

M02 5

N02 6

N02 6 1.000

N02 6

N02 6 5 0.101 28.448

N02 6 11 0.031 4.165

N02 6 27 0.038 2.104

N02 6 77 0.044 0.862

N02 6 3441 0.364 0.160

N02 6 6245 0.176 0.043

N02 6 7000 0.500 0.108

N02 6

O02 7 1.000

O02 7

O02 7 3 0.056 24.695

O02 7 5 0.065 18.654

O02 7 10 0.032 4.784

O02 7 15 0.015 1.513

O02 7 28 0.048 2.638

O02 7 78 0.051 0.995

O02 7 191 0.009 0.074

O02 7 1490 0.007 0.007

O02 7 1544 0.015 0.015

O02 7 1604 0.008 0.008

O02 7 3757 0.046 0.019

O02 7 6175 0.461 0.113

O02 7 7000 0.500 0.108

O02 7

(31)

A03 1A 93 0.006 0.099

A03 1A 143 0.012 0.122

A03 1A 3483 0.074 0.032

A03 1A 4749 0.438 0.140

A03 1A 7000 0.500 0.108

A03 1A

B03 2A 1.000

B03 2A

B03 2A 15 0.041 4.023

B03 2A 28 0.012 0.686

B03 2A 35 0.026 1.119

B03 2A 143 0.009 0.096

B03 2A 3341 0.054 0.025

B03 2A 4841 0.401 0.125

B03 2A 7000 0.500 0.108

B03 2A

C03 3A 1.000

C03 3A

C03 3A 15 0.039 4.007

C03 3A 28 0.020 1.069

C03 3A 34 0.028 1.265

C03 3A 36 0.014 0.571

C03 3A 3171 0.010 0.005

C03 3A 3406 0.025 0.011

C03 3A 4698 0.263 0.085

C03 3A 6107 0.187 0.046

C03 3A 7000 0.500 0.108

C03 3A

D03 4A 1.000

D03 4A 15 0.035 3.567

D03 4A 35 0.022 0.941

D03 4A 142 0.009 0.096

D03 4A 181 0.011 0.092

D03 4A 3330 0.020 0.009

D03 4A 4793 0.342 0.108

D03 4A 5733 0.031 0.008

D03 4A 7000 0.500 0.108

D03 4A

(32)

(ng/ul)

D03 4A

E03 5A 1.000

E03 5A

E03 5A 15 0.029 3.021

E03 5A 28 0.016 0.872

E03 5A 34 0.013 0.577

E03 5A 36 0.009 0.382

E03 5A 3599 0.065 0.027

E03 5A 4825 0.241 0.076

E03 5A 6214 0.163 0.040

E03 5A 7000 0.500 0.108

E03 5A

F03 6A 1.000

F03 6A

F03 6A 15 0.026 2.573

F03 6A 35 0.016 0.702

F03 6A 3397 0.243 0.109

F03 6A 4681 0.231 0.075

F03 6A 5941 0.076 0.019

F03 6A 7000 0.500 0.108

F03 6A

G03 7A 1.000

G03 7A

G03 7A 13 0.077 8.910

G03 7A 25 0.048 2.924

G03 7A 32 0.045 2.117

G03 7A 2560 0.117 0.069

G03 7A 2950 0.891 0.457

G03 7A 3943 0.093 0.036

G03 7A 5008 1.173 0.355

G03 7A 7000 0.500 0.108

G03 7A

(33)

H03 8A

I03 blank 1.000

I03 blank

I03 blank 2 0.191 132.162

I03 blank 10 0.033 4.845

I03 blank 14 0.038 4.052

I03 blank 26 0.057 3.292

I03 blank 70 0.014 0.304

I03 blank 76 0.081 1.600

I03 blank 2174 0.005 0.003

I03 blank 7000 0.500 0.108

I03 blank

J03 blank 1.000

J03 blank

J03 blank 2 0.158 109.405

J03 blank 11 0.033 4.654

J03 blank 14 0.040 4.162

J03 blank 27 0.065 3.687

J03 blank 71 0.014 0.294

J03 blank 76 0.088 1.746

J03 blank 7000 0.500 0.108

K03 blank 1.000

K03 blank

K03 blank 14 0.053 5.574

K03 blank 33 0.055 2.498

K03 blank 86 0.031 0.552

K03 blank 91 0.016 0.260

K03 blank 180 0.018 0.148

K03 blank 7000 0.500 0.108

L03 blank 1.000

L03 blank

L03 blank 14 0.139 14.747

L03 blank 33 0.090 4.175

L03 blank 85 0.040 0.706

L03 blank 90 0.025 0.427

L03 blank 177 0.045 0.387

L03 blank 6823 0.489 0.109

L03 blank 7000 0.500 0.108

(34)

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(36)

(37)

(38)

(39)

(40)

(41)

(42)

(43)

(44)

3. Experion results

See next pages for the results retrieved from the Experion experiments.

Several experiments is performed with this method. PDF documents are obtained from this, and are shown on the next pages. Blood and saliva samples were tested for the CYP2D6 deletion assay on the Experion technology.

Run overview report

1) Results of blood samples 2) Results of saliva samples

Primers NEW DEL – OLD GENE 3) Results of validation experiment

77 samples of another cohort

(45)

(46)

Page 2 of 14

Project: Prathima Acq. Analyst: DefaultUser

Assay: DNA 12K Acq. Time: 5/18/2015 1:04:31 PM

Run: Run_20150518_5-18-2015_1-04-29 PM Signature: N/A

Run Version: N/A

Virtual Gel Report

(47)

Well# Ladder

Peak Peak Mig. Time Size Corrected Area Concentration Molarity % Total Observation Comments

State Number (secs) (bp) Area Ratio (ng/µl) (nmole/l)

1 29.15 50 71.36 Ladder Peak Lower Marker

2 30.75 100 23.23 Ladder Peak

3 37.45 300 26.30 Ladder Peak

4 43.40 500 27.82 Ladder Peak

5 47.65 700 27.28 Ladder Peak

6 51.30 1,000 27.59 Ladder Peak

7 53.95 1,500 26.20 Ladder Peak

8 54.85 2,000 22.79 Ladder Peak

9 56.70 3,000 25.62 Ladder Peak

10 58.40 5,000 23.95 Ladder Peak

11 59.75 7,000 21.91 Ladder Peak

Well# Ladder

(48)

Page 4 of 14

Run Version: N/A

12 61.60 10,380 22.78 Ladder Peak

13 63.50 17,000 26.91 Ladder Peak Upper Marker

Well# Ladder

Egram, Gel Lane and Result Table Report

(49)

Well# 1 Sample 1

1 29.15 50 65.23 Lower Marker

2 58.83 5,633 6.36 0.2345 1.08 0.29 100.00

3 63.50 17,000 27.14 Upper Marker

Well# 1 Sample 1

Sample Peaks Found: 1 Total Concentration: 1.1 ng/µl TotalMolarity: 0.3 nmol/l

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Run Version: N/A

Well# 2 Sample 2

1 29.15 50 57.33 Lower Marker

2 58.75 5,516 9.77 0.4007 1.84 0.51 100.00

3 63.50 17,000 24.38 Upper Marker

Well# 2 Sample 2

Egram, Gel Lane and Result Table Report

(51)

Well# 3 Sample 3

1 29.15 50 51.86 Lower Marker

2 63.50 17,000 21.62 Upper Marker

Well# 3 Sample 3

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Run Version: N/A

Well# 4 Sample 4

1 29.15 50 45.23 Lower Marker

2 63.50 17,000 19.69 Upper Marker

Well# 4 Sample 4

Egram, Gel Lane and Result Table Report

(53)

Well# 5 Sample 5

1 29.15 50 38.91 Lower Marker

2 63.50 17,000 16.32 Upper Marker

Well# 5 Sample 5

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Run Version: N/A

Well# 6 Sample 6

1 29.15 50 34.25 Lower Marker

2 63.50 17,000 14.39 Upper Marker

Well# 6 Sample 6

Egram, Gel Lane and Result Table Report

(55)

Well# 7 Sample 7

1 29.15 50 28.78 Lower Marker

2 63.50 17,000 11.74 Upper Marker

Well# 7 Sample 7

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Run Version: N/A

Well# 8 Sample 8

1 29.15 50 26.23 Lower Marker

2 63.50 17,000 11.26 Upper Marker

Well# 8 Sample 8

Egram, Gel Lane and Result Table Report

(57)

Well# 9 Sample 9

1 29.15 50 23.74 Lower Marker

2 63.50 17,000 10.49 Upper Marker

Well# 9 Sample 9