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University of Groningen

Engineering endogenous hexose transporters in Saccharomyces cerevisiae for efficient

D-xylose transport

Nijland, Jeroen

IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from

it. Please check the document version below.

Document Version

Publisher's PDF, also known as Version of record

Publication date:

2019

Link to publication in University of Groningen/UMCG research database

Citation for published version (APA):

Nijland, J. (2019). Engineering endogenous hexose transporters in Saccharomyces cerevisiae for efficient

D-xylose transport. University of Groningen.

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ENGINEERING ENDOGENOUS

HEXOSE TRANSPORTERS

IN SACCHAROMYCES CEREVISIAE

FOR EFFICIENT D-XYLOSE TRANSPORT

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Engineering endogenous hexose

transporters in Saccharomyces

cerevisiae for efficient D-xylose

transport

Proefschrift

ter verkrijging van de graad van doctor aan de

Rijksuniversiteit Groningen

op gezag van de

rector magnificus prof. dr. E. Sterken

en volgens besluit van het College voor Promoties.

De openbare verdediging zal plaatsvinden op

vrijdag 1 maart 2019 om 16.15 uur

door

Jeroen G Nijland

geboren op 8 december 1972

te Borger

The work described in this thesis was carried out in the Molecular Microbiology

Group of the Groningen Biomolecular Sciences and Biotechnology Institute (GBB) of the University of Groningen, The Netherlands, and financially sup-ported by the EOS Long Term grant (EOS-LT) from the Dutch Ministry of Eco-nomic Affairs, Agriculture and Innovation, and by the research program of the bio-based ecologically balanced sustainable industrial chemistry (BE- BASIC).

ISBN (printed version): 978-94-034-1447-8 ISBN (electronic version): 978-94-034-1446-1 Original cover design: Julius, Luke and Ruben Nijland

Layout: Lovebird design.

www.lovebird-design.com Printing: Eikon +

Copyright © 2018 by Jeroen G Nijland. All rights reserved. No part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, without prior permission of the author.

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TABLE OF CONTENTS

Chapter 1: Introduction: Pentose transport in Saccharomyces

cerevisiae

7

Chapter 2: Increased xylose affinity of Hxt2 through gene

shuffling of hexose transporters in Saccharomyces

cerevisiae

31

Chapter 3: Improved xylose metabolism by a CYC8 mutant

of Saccharomyces cerevisiae

49

Chapter 4: Engineering of an endogenous hexose transporter

into a specific D-xylose transporter facilitates

glu-cose-xylose co-consumption in Saccharomyces

cerevisiae

83

Chapter 5: Improving pentose fermentation by preventing

ubiquitination of hexose transporters in

Saccha-romyces cerevisiae

113

Chapter 6: Improved D-xylose uptake and consumption in

an evolutionary engineered Saccharomyces

cer-evisiae strain

139

Bibliography

177

Summary

193

Samenvatting

199

Curriculum Vitae

205

List of publications and patents

207

Acknowledgements / Dankwoord

211

Promotor

Prof. dr. A.J.M. Driessen

Beoordelingscommissie

Prof. dr. M. Heinemann

Prof. dr. I.J. van der Klei

Prof. dr. P.J. Punt

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