Human virus-specific T cells in peripheral blood and lymph nodes: Phenotype, function and clonal relationships - Front matter

(1)

UvA-DARE is a service provided by the library of the University of Amsterdam (https://dare.uva.nl)

UvA-DARE (Digital Academic Repository)

Human virus-specific T cells in peripheral blood and lymph nodes: Phenotype,

function and clonal relationships

Remmerswaal, E.B.M.

Publication date

2014

Document Version

Final published version

Link to publication

Citation for published version (APA):

Remmerswaal, E. B. M. (2014). Human virus-specific T cells in peripheral blood and lymph

nodes: Phenotype, function and clonal relationships.

General rights

It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s)

and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open

content license (like Creative Commons).

Disclaimer/Complaints regulations

If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please

let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material

inaccessible and/or remove it from the website. Please Ask the Library: https://uba.uva.nl/en/contact, or a letter

to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You

will be contacted as soon as possible.

(2)

HUMAN VIRUS-SPECIFIC T CELLS IN

PERIPHERAL BLOOD AND LYMPH NODES:

phenotype, function and clonal relationships

H

U

MAN VI

R

US-SPE

C

IFIC T CELLS IN PE

R

IPHER

A

L

B

LOOD

A

ND

LYMPH NODES:

phenotype, fun

ct

ion and c

lona

l r

elat

ionsh

ip

s

Ester B.M. Remmerswaal

2014

(3)

HUMAN VIRUS-SPECIFIC T CELLS IN PERIPHERAL

BLOOD AND LYMPH NODES:

PHENOTYPE, FUNCTION AND CLONAL RELATIONSHIPS

(4)

(5)

(6)

This research was performed at the department of Experimental Immunology (EXIM) and the Renal Transplant Unit, Department of Internal Medicine, at the Academic Medical Centre (Amsterdam, the Netherlands).

Title: Human virus-specific T cells in peripheral blood and lymph nodes: phenotype, function and clonal relationships Cover: "verdedigingswerken", 2014

by Marjolein Spitteler (www.marjoleinspitteler.nl) Printing and layout: Off Page

ISBN: 978-94-6182-492-9

(7)

HUMAN VIRUS-SPECIFIC T CELLS IN PERIPHERAL

BLOOD AND LYMPH NODES:

PHENOTYPE, FUNCTION AND CLONAL RELATIONSHIPS

ACADEMISCH PROEFSCHRIFT

ter verkrijging van de graad van doctor aan de Universiteit van Amsterdam op gezag van de Rector Magnificus

prof. dr. D.C. van den Boom

ten overstaan van een door het college voor promoties ingestelde commissie, in het openbaar te verdedigen in de Agnietenkapel

op woensdag 19 november 2014, te 14:00 uur door

Elisabeth Bernardina Maria Remmerswaal geboren te Voorburg

(8)

Promotiecommissie

Promotores: Prof. Dr. R.A.W. van Lier Prof. Dr. R.J.M. ten Berge

Overige leden: Prof. Dr. A. Akbar Dr. V. Appay

Prof. Dr. T.B.H. Geijtenbeek Prof. Dr. T.W. Kuijpers Dr. G.M.G.M. Verjans Prof. Dr. H.L. Zaaijer

(9)

αβ

CD8+_{T cells}

Chapter 3 Two dimensions in human CD8+_{T-cell development:} ₆₃

cell surface phenotype in conjunction with T-bet and Eomes expression levels predicts the functional potential of antigen-experienced CD8+_T-cells

Chapter 4 Deep sequencing of antiviral T-cell responses to hCmv 97 and EBv in humans reveals a stable repertoire that is

maintained for many years

Chapter 5 Human virus-specific effector-type T cells accumulate 125 in blood but not in lymph nodes

Chapter 6 Clonal evolution of CD8+ _{T cell responses against latent} ₁₄₉

viruses: relationship between phenotype, localization and function

Chapter 7 Emergence of a CD28

⁻

granzyme B+_CD4+_{cytomegalovirus} ₁₈₃

-specific T cell subset after recovery of primary cytomegalovirus infection

Chapter 8 Strong selection of virus-specific cytotoxic CD4+_T-cell ₂₀₅

clones during primary human cytomegalovirus infection

Chapter 9 General discussion 225

Appendix Summary 241

Samenvatting voor niet ingewijden 245

List of Publications 253

Abbreviations 257

PhD portfolio 261

Curriculum vitae 263

Human virus-specific T cells in peripheral blood and lymph nodes: Phenotype, function and clonal relationships - Front matter

UvA-DARE (Digital Academic Repository)

Human virus-specific T cells in peripheral blood and lymph nodes: Phenotype,

function and clonal relationships

Remmerswaal, E.B.M.

Publication date

2014

Document Version

Final published version

Link to publication

Citation for published version (APA):

Remmerswaal, E. B. M. (2014). Human virus-specific T cells in peripheral blood and lymph

nodes: Phenotype, function and clonal relationships.

General rights

It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s)

and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open

content license (like Creative Commons).

Disclaimer/Complaints regulations

If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please

let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material

inaccessible and/or remove it from the website. Please Ask the Library: https://uba.uva.nl/en/contact, or a letter

to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You

will be contacted as soon as possible.

HUMAN VIRUS-SPECIFIC T CELLS IN

PERIPHERAL BLOOD AND LYMPH NODES:

phenotype, function and clonal relationships

H

U

MAN VI

R

US-SPE

C

IFIC T CELLS IN PE

R

IPHER

A

L

B

LOOD

A

ND

LYMPH NODES:

phenotype, fun

ct

ion and c

lona

l r

elat

ionsh

ip

s

Ester B.M. Remmerswaal

Ester B.M. Remmerswaal

2014

HUMAN VIRUS-SPECIFIC T CELLS IN PERIPHERAL

BLOOD AND LYMPH NODES:

PHENOTYPE, FUNCTION AND CLONAL RELATIONSHIPS

HUMAN VIRUS-SPECIFIC T CELLS IN PERIPHERAL

BLOOD AND LYMPH NODES:

PHENOTYPE, FUNCTION AND CLONAL RELATIONSHIPS

Promotiecommissie

TABLE OF CONTENTS

αβ

⁻