Application of microarray-based gene expression technology to neuromuscular disorders Sterrenburg, P.J.E.

Application of microarray-based gene expression

technology to neuromuscular disorders

Sterrenburg, P.J.E.

Citation

Sterrenburg, P. J. E. (2007, January 18). Application of microarray-based gene expression technology to neuromuscular disorders. Retrieved from https://hdl.handle.net/1887/8914

Version: Corrected Publisher’s Version

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List of publications

E. Sterrenburg, R. Turk, J.M. Boer, G.J.B. van Ommen and J.T. den Dunnen.

A common reference for cDNA microarray hybridizations.

Nucleic Acids Res. 2002 Nov 1;30(21):e116

S.J. White, E. Sterrenburg, G.J.B. van Ommen, J.T. den Dunnen and M.H. Breuning.

An alternative to FISH: detecting deletion and duplication carriers within 24 hours.

J Med. Genet. 2003 Oct;40(10):e113

P.A.C. ‘t Hoen, R. Turk, J.M. Boer, E. Sterrenburg, R.X. de Menezes, G.J.B. van Ommen and J.T. den Dunnen

Intensity-based analysis of two-colour microarrays enables effi cient and fl exible hybridization designs.

Nucleic Acids Res. 2004 Feb 24;32(4):e41

R. Turk, P.A.C. ‘t Hoen, E. Sterrenburg, R.X. de Menezes, E.J. de Meijer, J.M. Boer, G.J.B. van Ommen and J.T. den Dunnen

Gene expression variation between Mouse inbred strains.

BMC Genomics 2004 Aug 18;5(1):57

E. Sterrenburg, R. Turk, P.A.C. ’t Hoen, J.C. van Deutekom, J.M. Boer, G.J.B. van Ommen and J.T. den Dunnen

Large-scale gene expression analysis of human skeletal myoblast differentiation.

Neuromuscul. Disord. 2004 Sep;14(8-9):507-18

R.Turk, E. Sterrenburg, E.J. de Meijer, G.J.B. van Ommen, J.T. den Dunnen, P.A.C. ’t Hoen

Muscle regeneration in dystrophin-defi cient mdx mice studied by gene expression profi ling.

BMC Genomics 2005 Jul 13;6:98

R. Turk, E. Sterrenburg, C.G. van der Wees, E.J. de Meijer, R.X. de Menezes, S. Groh, K.P. Campbell, S. Noguchi, G.J.B. van Ommen, J.T. den Dunnen, P.A.C. ’t Hoen Common pathological mechanisms in mouse models for muscular dystrophies.

FASEB J. 2006 Jan;20(1):127-9

E. Sterrenburg, C.G. van der Wees, S.J. White, R. Turk, R.X. de Menezes, G.J.B. van Ommen, J.T. den Dunnen, P.A.C. ’t Hoen

Gene expression profi ling highlights defective myogenesis in DMD patients and a possible role for bone morphogenetic protein 4.

Neurobiol. Dis. 2006 Jul;23(1):228-36

E. Sterrenburg, S.J.E. Routledge, B.M. van der Sluijs, C.G.C. van der Wees, H.G. ter Laak, B. G.M. van Engelen, S.M. van der Maarel and M. Antoniou

A skeletal muscle cell model of oculopharyngeal muscular dystrophy reveals an extracellular matrix defect.

Submitted

List of publications

Curriculum Vitae

De auteur van dit proefschrift werd geboren op 22 februari 1977 in Eindhoven. In 1995 behaalde zij haar VWO diploma aan het Anton van Duinkerkencollege te Veldhoven. In datzelfde jaar begon zij met de studie Biologische gezondheidkunde aan de Universiteit van Maastricht. Tijdens haar studie liep ze stage bij de afdelingen Toxicologie (dr. Geja Hageman) en Klinische Genetica (dr. Marian Stevens-Kroef). Haar literatuurscriptie schreef zij onder supervisie van dr. John Engelen met als onderwerp X-chromosoom inactivatie. Het doctoraal examen werd behaald in 1999 waarna gestart werd met het in dit proefschrift beschreven promotie onderzoek onder begeleiding van dr. Johan den Dunnen, prof. dr. Silvère van der Maarel en prof. dr. Gert-Jan van Ommen op de afdeling Humane Genetica van het Leids Universitair Medisch Centrum. Sinds januari 2005 is zij werkzaam als post-doc op dezelfde afdeling op een door de EU gefi nancierd project getiteld: ‘Insights to novel therapeutic strategies for a nuclear inclusion disease caused by polyalanine expansion’.

Curriculum Vitae

Chapter 3, Figure 1, page 56

Fig. 1. Immunohistochemical staining of myoblasts/myotubes. Cells of the KM109 cell culture in different stages of myogenesis (time in days after serum deprivation) were stained with DAPI (blue) and antibodies to desmin (red) and myosin (green). In this culture, 85% of the cells were desmin-positive, indicating myogenic potential. Multinucleated, myosin- positive cells can be seen from day 4 (arrow).

Chapter 3, Figure 3, page 62

Fig. 3. Hierarchical clustering of genes up- (red) and down-(green) regulated in time (t¼ 0; t ¼ 1; t ¼ 2; t ¼ 4 and t ¼ 14) according to cDNA arrays hybridized with three primary human muscle cell cultures (1¼ KM109, 2 ¼ KM108, 3 ¼ HPP4). Rows are differentially expressed genes. Columns are individual cell cultures.

Appendix

Chapter 4, Figure 1, page 72

Fig. 2. Immunohistochemical staining of healthy and DMD myotubes incubated with different concentrations of BMP4, evaluated on day 7 of differentiation.

Cells were stained with DAPI (blue) and antibodies to desmin (red) and myosin (green).