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Cover Page The handle http://hdl.handle.net/1887/35908 holds various files of this Leiden University dissertation

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Cover Page

The handle http://hdl.handle.net/1887/35908 holds various files of this Leiden University dissertation

Author: Soema, Peter

Title: Formulation of influenza T cell peptides : in search of a universal influenza vaccine

Issue Date: 2015-10-20

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Formulation of influenza T cell peptides:

In search of a universal influenza vaccine

Peter Soema

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ISBN: 978-94-6233-085-6

© 2015, Peter Soema. All rights reserved. No parts of this thesis may be reproduced or transmitted in any form or by any means without permission of the author.

The research in this thesis was performed at the department of Research of the Institute of Translational Vaccinology (Intravacc), Bilthoven, The Netherlands.

Parts of the research described in this thesis was supported by the Center for Translational Molecular Medicine grant AMPVACS.

Printing of this thesis was financially supported by the Institute of Translational Vaccinology (Intravacc).

About the cover: A combined graphic of a T cell peptide and the three adjuvants central in this thesis: whole inactivated influenza virus, virosomes and liposomes.

Printed by: Gildeprint

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Formulation of influenza T cell peptides:

In search of a universal influenza vaccine

Proefschrift

ter verkrijging van

de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof. mr. C.J.J.M. Stolker,

volgens besluit van het College voor Promoties te verdedigen op dinsdag 20 oktober 2015

klokke 15.00 uur door

Peter Christiaan Soema geboren te Den Haag

in 1987

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Promotiecommissie

Promotoren:

Prof. dr. G.F.A. Kersten Prof. dr. W. Jiskoot Co-promotor:

Dr. J-P. Amorij (Intravacc) Overige leden:

Prof. dr. M. Danhof

Prof. dr. A. Huckriede (Rijksuniversiteit Groningen) Prof. dr. F. Ossendorp

Dr. E. Mastrobattista (Universiteit Utrecht)

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TAbLE OF COnTEnTS

Chapter 1

General introduction and thesis outline ...

7

Chapter 2

Current and next generation influenza vaccines: Formulation and production strategies ...

13

Chapter 3

Influenza T cell epitope-loaded virosomes adjuvanted with CpG as a potential influenza vaccine ...

53

Chapter 4

Whole inactivated influenza virus as an adjuvant for influenza peptide antigens ...

75

Chapter 5

Predicting the influence of liposomal lipid composition on liposome size, zeta potential and liposome-induced dendritic cell maturation using a design of experiments approach ...

97

Chapter 6

Development of cross-protective influenza A vaccines based on cellular responses ...

119

Chapter 7

Solid bioneedle-delivered influenza vaccines are highly thermostable and induce both humoral and cellular immune responses ...

145

Chapter 8

Summary and perspectives ...

169

Appendix

nederlandse samenvatting ...

180

List of publications ...

188

Curriculum vitae ...

189

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