The effect of bacterial isochorismate synthase on the Brassica rapa metabolome
Simoh, S.
Citation
Simoh, S. (2008, June 11). The effect of bacterial isochorismate synthase on the Brassica rapa metabolome. Retrieved from https://hdl.handle.net/1887/12944
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The effect of bacterial isochorismate synthase
on the Brassica rapa metabolome
Sanimah Simoh
The effect of bacterial isochorismate synthase on the Brassica rapa metabolome
ISBN 978-90-9023055-9
Printed by PrintPartners Ipskamp B. V., Amsterdam, The Netherlands
Cover: Brassica rapa (artist: Zina Deretsky; Courtesy: National Science Foundation)
The effect of bacterial isochorismate synthase on the Brassica rapa metabolome
PROEFSCHRIFT
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van Rector Magnificus prof. mr. P. F. van der Heijden, volgens besluit van het College voor Promoties
te verdedigen op woensdag 11 juni 2008 klokke 13.45 uur
door
Sanimah Simoh
geboren te Johore (Maleisië) in 1970
Promotiecommissie
Promotor Prof. dr. R. Verpoorte
Co-promotor Dr. H. J. M. Linthorst
Referent Prof. dr. L.H.W. van der Plas (Wageningen Universiteit)
Overige Leden Prof. dr. P. J. J. Hooykaas
Prof. dr. C. A. M. J. J. van den Hondel
Dr. A. E. Schulte
Contents
Abbreviations iv
Chapter 1 General Introduction Error! Bookmark not defined.
Chapter 2 Host-bacterium interactions in Agrobacterium tumefaciens-mediated plant transformation: Mechanism of action and Agrobacterium/plant factors involved (review) Error! Bookmark not defined.
Chapter 3 Agrobacterium-mediated transformation of Brassica rapa with a bacterial isochorismate synthase (ICS) gene 31 Chapter 4 Salicylic acid production in transgenic Brassica rapa transformed
with a gene encoding bacterial isochorismate synthase 53 Chapter 5 Phylloquinone (vitamin K1) and glucosinolate contents in transgenic
Brassica rapa transformed with a bacterial isochorismate synthase
gene 65
Chapter 6 Metabolome analysis of Brassica rapa transformed with a bacterial
gene encoding isochorismate synthase 77
Chapter 7 1H NMR analysis of metabolomic changes in Brassica rapa upon infection with Agrobacterium tumefaciens 93 Chapter 8 Future perspective in engineering plant secondary metabolism 109
Summary 113
Samenvatting 117
References 121
Curriculum Vitae 137
Acknowledgements 138
Abbreviations
ABA abscisic acid
AS acetosyringone
ATP adenosine triphospate
BA benzoic acid
BAP benzyl amino purine
CA cinnamic acid
CaMV cauliflower mosaic virus COSY correlated spectroscopy
CTAB hexadecyltrimethylammonium bromide DEAE diethylaminoethyl
DNA deoxyribonucleic acid
dNTP deoxyribonucleotide triphosphate DSB double strand break
EDTA ethylenediamine tetraacetate
GC gas chromatography
GE genetic engineering GFP green fluorescence protein GLS glucosinolate
GTP guanosine triphosphate
HMBC heteronuclear multiple-bond correlation HPLC High Performance Liquid Chromatography HR homologous recombination
HSQC heteronuclear single-quantum coherence IAA indole 3-acetic acid
ICS isochorismate synthase IPL isochorismate pyruvate lyase LAR local acquired resistant
LB Left Border
LB medium Luria Betani medium
LC-MS liquid chromatography mass spectrometry M&S Murashige & Skoog
mRNA messenger ribonucleic acid NAA naphtaleneacetic acid NHEJ non homologous end joining NHR non-homologous recombination NLS nuclear localization signal
NMR nuclear magnetic resonance PCA principal component analysis PCR polymerase chain reaction PIt proteinase inhibitor terminator PLS partial least square regression
PLS-DA partial least square-discriminant analysis PR pathogenesis related protein
RB Right Border
RNA ribonucleic acid
ROS reactive oxygen species
RT-PCR reversed transcription-polymerase chain reaction SA salicylic acid
SAAT sonication assisted Agrobacterium-mediated transformation SAG salicylic acid glucoside
SAR systemic acquired resistance SDS sodium dodecyl sulfate ssT-DNA single stranded T-DNA T4SS type 4 secretion systems TCA trichloroacetic acid TMV tobacco mosaic virus UV-B ultra violet B
