University of Groningen
Poor old pores
Rempel, Irina Lucia
IMPORTANT NOTE: You are advised to consult the publisher's version (publisher's PDF) if you wish to cite from it. Please check the document version below.
Document Version
Publisher's PDF, also known as Version of record
Publication date: 2019
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Rempel, I. L. (2019). Poor old pores: The cell’s challenge to make and maintain nuclear pore complexes in aging. University of Groningen.
Copyright
Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).
Take-down policy
If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.
Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum.
Poor old pores
The cell’s challenge to make and maintain
nuclear pore complexes in aging
Cover: Yeast cells are going for a run in the microfluidic chip Cover design: Irina Lucia Rempel
Printed by: Optima Grafische Communicatie B.V. – The Netherlands ISBN (print version): 978-94-034-1818-6
ISBN (digital version): 978-94-034-1819-3 Copyright © by Irina Lucia Rempel
All rights reserved. No parts of this book may be reproduced or transmitted in any form or by any means without prior permission of the author.
Cover: Yeast cells are going for a run in the microfluidic chip Cover design: Irina Lucia Rempel
Printed by: Optima Grafische Communicatie B.V. – The Netherlands ISBN (print version): 978-94-034-1818-6
ISBN (digital version): 978-94-034-1819-3 Copyright © by Irina Lucia Rempel
All rights reserved. No parts of this book may be reproduced or transmitted in any form or by any means without prior permission of the author.
Poor old pores
The cell’s challenge to make and maintain nuclear pore complexes in aging
Phd thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus prof. E. Sterken
and in accordance with the decision by the College of Deans. This thesis will be defended in public on
Tuesday 9 July 2019 at 9.00 hours
by
Irina Lucia Rempel
born on 19 June 1988 in Hamburg, Germany
Supervisors
Dr. L.M. Veenhoff Prof. E.A.A. Nollen
Co-supervisor
Dr. M. Chang
Assessment Committee
Prof. H.H. Kampinga Prof. M.P. Rout Prof. E.M.J. Verpoorte
Supervisors
Dr. L.M. Veenhoff Prof. E.A.A. Nollen
Co-supervisor
Dr. M. Chang
Assessment Committee
Prof. H.H. Kampinga Prof. M.P. Rout Prof. E.M.J. Verpoorte
To the people that encouraged me to finish high school (Gymnasium) and go to university…
Contents
Chapter 1
A general introduction into aging and nuclear pore complexes in baker’s
yeast
9
Chapter 2
Age-dependent deterioration of nuclear pore assembly in mitotic cells
decreases transport dynamics
33
Chapter 3
Flexible and extended linker domains support efficient targeting of
Heh2 to the inner nuclear membrane
73
Chapter 4
Replicative and chronological aging differently impact nuclear transport
in baker’s yeast
101
Chapter 5
Poor old pores – The challenge of making and maintaining nuclear pore
complexes in aging
119
Bibliography
