University of Groningen
Quantification of macromolecular crowding and ionic strength in living cells
Liu, Boqun
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2018
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Liu, B. (2018). Quantification of macromolecular crowding and ionic strength in living cells. Rijksuniversiteit
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Quantification
of macromolecular
crowding
and ionic strength
in Living cells
Quantification of macromolecular crowding and ionic strength in Living cells Academic Thesis, University of Groningen, the Netherlands
The work published in this thesis was carried out in the Membrane Enzymology group of the Biochemistry Department of the University of Groningen, the Netherland. This work was financially supported by the China Scholarship Council grant, ERC Advanced Grant, and Netherlands Organization for Scientific Research Vidi grant.
ISBN: 978-94-034-0718-0 (printed book) 978-94-034-0717-3 (ebook) Printing: Eikon +
Cover & layout: Lovebird design. www.lovebird-design.com
© B. Liu, Groningen, the Netherlands, 2018
All rights reserved. No part of this publication may be reproduced or transmitted in any form or by any means, without written permission of the author.
Quantification of macromolecular crowding and
ionic strength in Living cells
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. E. Sterken
and in accordance with the decision by the College of Deans. This thesis will be defended in public on
Friday 1 June 2018 at 12.45 hours
by
Boqun Liu
born on 2 September 1987 in Jilin, China Supervisor Prof. B. PoolmanCo-supervisor Dr. A.J. Boersma Assessment Committee Prof. G. Maglia Prof. M. Heinemann Prof. P. Swain
Quantification of macromolecular crowding and
ionic strength in Living cells
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. E. Sterken
and in accordance with the decision by the College of Deans. This thesis will be defended in public on
Friday 1 June 2018 at 12.45 hours
by
Boqun Liu
born on 2 September 1987 in Jilin, China Supervisor Prof. B. PoolmanTable of contents
CHAPTER 1
Quantification of macromolecular crowding in the intracellular environment ... 7
CHAPTER 2
Design and Properties of Genetically-Encoded Probes for Sensing Macromo-lecular Crowding ...29Supporting information: Design and Properties of Genetically-Encoded Probes for Sensing Macromolecular Crowding ...53
Chapter 3
Comparison of fluorescent proteins in a crowding sensor and the importance of efficient maturation in Escherichia coli ...69Chapter 4
Macromolecular crowding during adaptation to hyperosmotic stress ...91Chapter 5
Ionic strength sensing in living cells ... 111Supporting information: Ionic strength sensing in living cells ... 123
Summary ... 135
Samenvatting ... 141
