Role of fibroblast-like synoviocytes in cartilage degradation during rheumatoid arthritis Tolboom, Tanja

Role of fibroblast-like synoviocytes in cartilage

degradation during rheumatoid arthritis

Tolboom, Tanja

Citation

Tolboom, T. (2005, October 20). Role of fibroblast-like

synoviocytes in cartilage degradation during rheumatoid

arthritis. Retrieved from https://hdl.handle.net/1887/4296

Version:

Corrected Publisher’s Version

License:

Licence agreement concerning inclusion

of doctoral thesis in the Institutional

Repository of the University of Leiden

Downloaded from:

https://hdl.handle.net/1887/4296

Note: To cite this publication please use the final published

 VSOLWLQDUDWLR)/6RISDVVDJHRUZHUHXVHGIRUDOOH[SHULPHQWV/LJKWPL FURVFRS\DQG*LHPVDVWDLQLQJLQGLFDWHGWKDWPRUHWKDQRIFHOOVZHUH)/6 ([SUHVVLRQDQGSXULÀFDWLRQRISURWHLQV 7KHFORQLQJH[SUHVVLRQDQGSXULÀFDWLRQRIUHFRPELQDQWPDOWRVHELQGLQJ SURWHLQ0%3$SRSWLQSURWHLQLWVFRQWUROSURWHLQ0%3DQGUHFRPELQDQW+LV WDJJHG$SRSWLQSURWHLQKDYHEHHQGHVFULEHGSUHYLRXVO\,QVKRUWWKH FRQVWUXFWS0DO7%93HQFRGHV0%3$SRSWLQDQ1WHUPLQDOIXVLRQRI0%3 WR$SRSWLQVHSDUDWHGE\DÁH[LEOHOLQNHUHQFRGLQJDVSDUDJLQHUHVLGXHV 7KHFRQVWUXFWS0DO7%HQFRGHV0%3DORQH%RWKSURWHLQVZHUHH[SUHVVHGLQ (VFKHULFKLDFROLDQGSXULÀHGE\VWDQGDUGPHWKRGV7KHSXULW\RIWKHSURWHLQV ZHUHJHQHUDOO\!,QWKHFDVHRI+LV$SRSWLQSXULÀFDWLRQZDVSHUIRUPHG RQ1L17$FROXPQVDVGHVFULEHG  7UDQVGXFWLRQRIFHOOVZLWKDSRSWLQSURWHLQ 2QWKHGD\EHIRUHWUDQVGXFWLRQWKHFHOOVZHUHSODWHGRQXQFRDWHGPPJODVV ERWWRPPLFURZHOOGLVKHV0DWWHN$VKODQG86$DWDGHQVLW\RIFHOOV 'XULQJPLFURLQMHFWLRQFHOOVZHUHLQFXEDWHGLQ530,PHGLXPP0 +(3(6S+IHWDOFDOIVHUXPSHQLFLOOLQDQGVWUHSWRP\FLQDWƒ&&HOOV ZHUHUHWXUQHGWR,0'0PHGLXPLPPHGLDWHO\DIWHUPLFURLQMHFWLRQ0LFURLQ  MHFWLRQZDVSHUIRUPHGZLWK—PPLFURQHHGOHVVWHULOHIHPWRWLSV,,(SSHQ  GRUIXQGHUDQLQYHUWHGPLFURVFRSH$[LRYHUW79=HLVVHTXLSSHGZLWK DSURJUDPPDEOHPLFURLQMHFWRU,01DULVKLJH&RDQGDMR\VWLFNK\GUDX  OLFPLFURPDQLSXODWRU0021DULVKLJH&R)OXRUHVFHLQODEHOHGFURVV OLQNHG0%3$SRSWLQPJPOLQ3%6ZDVFRPLFURLQMHFWHGZLWKO\VLQHÀ[  DEOHUKRGDPLQHGH[WUDQ0ROHFXODU3UREHVWKDWZDVXVHGDVDPDUNHUWRWUDFH LQMHFWHGFHOOV'LUHFWO\SULRUWRLQMHFWLRQSURWHLQVDPSOHVZHUHÀOWHUHGRYHU —PPLFURÀOWHUWXEHPLOOLSRUHRUDWðJFHQWULIXJDWLRQIRUPLQ0LFUR LQMHFWLRQZDVSUHIRUPHGXQGHUWKHFRQGLWLRQRILQMHFWLRQSUHVVXUHRIWR SRXQGVVTXDUHLQFKDQGDQLQMHFWLRQWLPHRIWRV)RUHDFKH[SHULPHQW FHOOVZHUHLQMHFWHGSHUGLVK$IWHUKRXUVRILQFXEDWLRQDW R&DQG

&2_WKHFHOOVZHUHZDVKHGZLWK3%6À[HGZLWKDFHWRQHLQ+ _2IRU

 FRQWDLQVWZRGLIIHUHQWGRPDLQVWKDWKDYHWKHDELOLW\WRLQGXFHDSRSWRVLVLQGH  SHQGHQWO\2QO\RQHGRPDLQVHHPVWREHUHJXODWHGE\SKRVSKRU\ODWLRQ7KHUH IRUHLWVHHPVOLNHO\WKDWRQO\RQHRIWKH$SRSWLQDSRSWRWLFGRPDLQVLVDFWLYDWHG LQ5$)/6$SRVVLELOLW\IRUIXUWKHUUHVHDUFKPD\EHWRH[SORUHWKHPHFKDQLVP E\LQYHVWLJDWLQJZKHWKHUHJFDVSDVHVDQGGHDWKHIIHFWRUGRPDLQDVVRFLDWHG IDFWRU'('$)ZKLFKDUHLQYROYHGLQDSRSWLQLQGXFHGDSRSWRVLVDUH DOVRLQYROYHGLQ5$)/6 7DEOH$SRSWLQVHQVLWLYLW\RIVHYHUDOKXPDQFHOOOLQHV)RUUHIHUHQFHVVHHWH[W &HOOOLQH $SRSWLQVHQVLWLYH &HOOW\SH