Function and control of the ssg genes in streptomyces
Traag, B.A.
Citation
Traag, B. A. (2008, September 24). Function and control of the ssg genes in streptomyces.
Retrieved from https://hdl.handle.net/1887/13114
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Function and control of the ssg genes in Streptomyces
PROEFSCHRIFT
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van Rector Magnificus prof. mr. P. F. van der Heijden, volgens besluit van het College voor Promoties
te verdedigen op 24 september 2008 klokke 13.45 uur
door
Bjørn Alwin Traag Geboren te Den Haag,
11 januari 1980
Promotiecommissie:
Promotor: Prof. dr. C.W.A. Pleij Co-promotor: Dr. G.P. van Wezel Referent: Dr. B. Kraal Overige leden: Prof. dr. J. Brouwer
Prof. dr. M.H.M. Noteborn
Prof. dr. C.A.M.J.J. van den Hondel Prof. dr. S.R. Douthwaite
Printed by Ponsen & Looijen BV, Wageningen, the Netherlands
Contents
Chapter I 5
Introduction
Chapter II 9
The SsgA-like proteins in actinomycetes: small proteins up to a big task
Chapter III 29
Transcription of the sporulation gene ssgA is activated by the IclR-type regulator SsgR in a whi-independent manner in
Streptomyces coelicolor A3(2)
Chapter IV 59
Control of Streptomyces differentiation through the regulation of SALPs
Chapter V 85
Characterization of the sporulation control protein SsgA by use of an efficient method to create and screen random mutant libraries in streptomycetes
Chapter VI 109
Functional and phylogenetic studies of the developmental cell division control protein SsgB in actinomycetes
Chapter VII
Summary and discussion 132
Nederlandse samenvatting 139
References 143
List of abbreviations 150
Appendices
Appendix A. Colour figures 152 Appendix B. The predicted structure of S. coelicolor SsgA 157
Acknowledgements 158
Curriculum vitae 159
