Control of sporulation-specific cell division in Streptomyces coelicolor
Noens, E.
Citation
Noens, E. (2007, September 25). Control of sporulation-specific cell division in
Streptomyces coelicolor. Department Microbial Development (LIC) Department Electron Microscopy (LUMC/MCB), Leiden University. Retrieved from
https://hdl.handle.net/1887/12351
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
Downloaded from: https://hdl.handle.net/1887/12351
Note: To cite this publication please use the final published version (if applicable).
Control of sporulation-specific
cell division
in Streptomyces coelicolor
Elke Elza Eduarda Noens
Control of sporulation-specific
cell division
in Streptomyces coelicolor
PROEFSCHRIFT
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van Rector Magnificus prof. mr. P. F. van der Heijden,
volgens besluit van het College voor Promoties
te verdedigen op dinsdag 25 september 2007
klokke 15.00 uur
door
Elke Elza Eduarda Noens
geboren te Kapellen, België
op 3 april 1979
PROMOTIECOMMISSIE
Promotor Prof. dr. C.W.A. Pleij
Co-Promotor Dr. H.K. Koerten
Dr. G.P. van Wezel
Referent Prof. dr. H.A.B. Wösten (Universiteit Utrecht)
Overige Leden Prof. dr. J. Brouwer Prof. dr. J.P. Abrahams Prof. dr. H.P. Spaink
Printing of this thesis was financially supported by the Stichting tot Bevordering van de Electronenmicroscopie in Nederland and the J.E. Jurriaanse Stichting.
Printed by Ponsen & Looijen BV, Wageningen, the Netherlands
“Do not go where the path may lead, go instead where there is no path and leave a trail.”
Ralph Waldo Emerson
CONTENTS
Chapter 1 Cell division during growth and development - the cytoskeleton
9
Chapter 2 SsgA-like proteins determine the fate of peptidoglycan during sporulation of Streptomyces coelicolor
35
Chapter 3 Analysis of cell division in the ssg mutants highlights SsgB and SsgG as important control proteins for the initiation of septum formation
63
Chapter 4 Loss of the controlled localisation of growth stage-specific cell wall synthesis pleiotropically affects developmental gene expression in an ssgA mutant of Streptomyces coelicolor
85
Chapter 5 MreBCD and Mbl of Streptomyces coelicolor are required for the integrity of aerial hyphae and spores
111
Chapter 6 FtsX en FtsE import autolytically produced peptidoglycan subunits during sporulation-specific cell division of Streptomyces coelicolor
141
Chapter 7 Summary and discussion Nederlandse samenvatting
159 166
Appendices Full colour images 171
References 186
Curriculum vitae 192
List of publications 192
