Unraveling the stepwise activation mechanism of HacA, the key regulator of the unfolded protein response in Aspergillus niger
Mulder, H.J.
Citation
Mulder, H. J. (2010, February 2). Unraveling the stepwise activation mechanism of HacA, the key regulator of the unfolded protein response in Aspergillus niger. Retrieved from https://hdl.handle.net/1887/14647
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
Downloaded from: https://hdl.handle.net/1887/14647
Note: To cite this publication please use the final published version (if applicable).
UnravellingthestepwiseactivationmechanismofHacA,
thekeyregulatoroftheUnfoldedProteinResponsein
Aspergillusniger
HarmMulder
The work described in this thesis was carried out at Danisco Genencor within
framework 5 of Eurofung, and was supported by EC Grant No: QLRK300729.
PublicationofthisthesiswasfinanciallysupportedbyDaniscoGenencor.
Printedby:IpskampDrukkers
ISBN:9789090249773
UnravellingthestepwiseactivationmechanismofHacA,
thekeyregulatoroftheUnfoldedProteinResponsein
Aspergillusniger
Proefschrift
terverkrijgingvan
degraadvanDoctoraandeUniversiteitLeiden,
opgezagvanRectorMagnificusprof.mr.P.F.vanderHeijden,
volgensbesluitvanhetCollegevoorPromoties
teverdedigenopdinsdag2februari2010
klokke15:00uur
door
HarmJanMulder
geborenteOosterendTexel
in1970
PromotionCommittee
Promoter: Prof.Dr.C.A.M.J.J.vandenHondel
Copromoter: Dr.J.Visser
OtherMembers: Prof.Dr.S.M.VerduynLunel
Prof.Dr.P.J.J.Hooykaas
Prof.Dr.P.J.Punt
Dr.A.F.J.Ram
Prof.Dr.D.B.Archer(UniversityofNottingham)
Dr.C.M.J.Sagt(DSM)
VoorSjuttjeenKorneliusJan
Contents
Chapter1
Chapter2
Chapter3
Chapter4
Chapter5
Summary
Samenvatting
References
Publications
Curriculumvitae
GeneralIntroduction
TheTranscriptionFactorHacAMediatestheUnfolded
ProteinResponseinAspergillusniger,andUpRegulates
itsOwnTranscription
HacA,theTranscriptionalActivatoroftheUnfolded
ProteinResponseinAspergillusniger,BindstoPartly
PalindromicUPRElementsoftheConsensusSequence
5'CAN(G/A)NTGT/GCCT3'
AHacADependentTranscriptionalSwitchReleases
hacAmRNAfromaTranslationalBlockupon
EndoplasmicReticulumStress
GeneralDiscussion
10
39
59
83
109
123
125
129
143
145
