Computational modeling indicates anaerobic glycogenolytic ATP synthesis contributes little to quadriceps energy balance during exhaustive bicycling exercise

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Computational modeling indicates anaerobic glycogenolytic

ATP synthesis contributes little to quadriceps energy balance

during exhaustive bicycling exercise

Citation for published version (APA):

Jeneson, J. A. L., Schmitz, J. P. J., Riel, van, N. A. W., Groenendaal, W., Nicolaij, K., & Hilbers, P. A. J. (2008).

Computational modeling indicates anaerobic glycogenolytic ATP synthesis contributes little to quadriceps energy

balance during exhaustive bicycling exercise. In Experimental biology 2008, San Diego California (pp. 756.1-).

(FASEB Journal : The Journal of the Federation of American Societies for Experimental Biology; Vol. 22).

FASEB. https://doi.org/10.1096/fj.08-0101ufm

DOI:

10.1096/fj.08-0101ufm

Document status and date:

Published: 01/01/2008

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756.1 Computational modeling indicates anaerobic

glycogenolytic ATP synthesis contributes little to

quadriceps energy balance during exhaustive

bicycling exercise

Jeroen Jeneson1_{, Joep Schmitz}1,2_{, Natal van Riel}2_{, Willemijn Groenendaal}2_{, Klaas} Nicolay1_{and Peter Hilbers}2

1_{Biomedical NMR}

2_{BioModeling and Bioinformatics Group, Department of Biomedical Engineering, Eindhoven}

University of Technology, Eindhoven, Netherlands

ABSTRACT

It has been proposed that anaerobic glycogenolysis may contribute as much as 20% to cellular ATP synthesis during contractile work on basis of indirect evidence from 31_{P NMR spectroscopic measurements of proton balance in human limb} muscles (1). Here, this hypothesis was tested more rigorously using a systems biology approach. We constructed a computational model of energy metabolism in muscle composed of slow-twitch oxidative, fast-twitch oxidative and fast-twitch glycolytic fibers incorporating mitochondrial and glycogenolytic ATP synthetic pathways operating in parallel. Next, we measured the in vivo dynamics of phosphocreatine (PCr), Pi, hexose monophosphates (HMP) and H+_{concentrations} in quadriceps muscle of human subjects performing in-magnet bicycling exercise using gated 31P NMR spectroscopy (2). The measured PCr dynamics were then compared to model simulations for different magnitudes of glycogenolytic ATP synthetic flux. In contrast to previous indirect analyses (1), we found that anaerobic ATP synthesis contributions of only 10% or less sufficed to explain energy balance during exercise; this in spite of massive activation of glycogenolysis as evidenced by large accumulations of HMP of up to 10 mM directly following exercise (2).

REFERENCES

• Kemper, et al. Proc Natl Acad Sci USA 2001 28756.1 • Jeneson, Schmitz and Nicolay (this meeting)

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