University of Groningen
Mom's sweet gift
Cheng, Lianghui
DOI:10.33612/diss.127480932
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Publication date: 2020
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Cheng, L. (2020). Mom's sweet gift: The beneficial effects of human milk oligosaccharides and their structure-function relationships. University of Groningen. https://doi.org/10.33612/diss.127480932
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Mom’s sweet gift
The beneficial effects of human milk oligosaccharides and their
structure-function relationships
This research described in this thesis was financially supported by the China Scholarship Council and University Medical Center Gronigen.
Final support by the University of Groningen, University Medical Center Gronigen, and the Graduate School of Medical Sciences (GUIDE) for the printing of this thesis is greatfully acknowleadged.
Layout Lianghui Cheng Cover design W.Qin
Printed by Off page
Amsterdam, The Netherlands ISBN 978-94-034-2655-6 (print)
978-94-034-2654-9 (digital)
© Copyright 2020 Lianghui Cheng, Groningen, The Netherdands
All right reserved. No part of the thesis may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, without prior written permission of the authour.
Mom’s sweet gift
The beneficial effects of human milk oligosaccharides and their
structure-function relationships
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. C. Wijmenga
and in accordance with the decision by the College of Deans. This thesis will be defended in public on
Tuesday 23 June 2020 at 11.00 hours
by
Lianghui Cheng
born on 24 October 1991Supervisor
Prof. P. de VosCo-supervisor
Dr. M.M. FaasAssessment Committee
Prof. G. Folkerts Prof. T. Plosch Prof. L. DijkhuizenTable of contents
Chapter 1 More than sugar in the milk: human milk oligosaccharides as essential bioactive molecules in breast milk and current insight in beneficial effects
9
Rationale and outline of the thesis 32
Chapter 2 Human milk oligosaccharides and its acid hydrolysate LNT2 show immunomodulatory effects via TLRs in a dose and structure-dependent way
39
Chapter 3 The human milk oligosaccharides 3-FL, LNnT, and LDFT attenuate TNF-α induced inflammation in fetal intestinal epithelial cells through shedding or interacting with TNF receptor 1
65
Chapter 4 Human milk oligosaccharides differently modulate goblet cells under homeostatic, proinflammatory conditions and under ER-stress
83
Chapter 5 Human milk oligosaccharides (hMOs) modulate the growth and fermentation of Bifidobacterium longum subsp. infantis in a hMO specific fashion in both monoculture and co-culture with Faecalibacterium prausnitzii
107
Chapter 6 Effects of human milk oligosaccharides on the crosstalk between commensal bacteria and intestinal epithelium in presence of intestinal peristaltic shear force
125
Chapter 7 General discussion 151 Appendices
Summary 164
Nederlandse samenvatting 168
Acknowledgments 172
