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Cover Page The handle http://hdl.handle.net/1887/55262 holds various files of this Leiden University dissertation. Author: Pawlak, J.B. Title: Bioorthogonal Antigens Issue Date: 2017-11-14

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Cover Page

The handle http://hdl.handle.net/1887/55262 holds various files of this Leiden University dissertation.

Author: Pawlak, J.B.

Title: Bioorthogonal Antigens

Issue Date: 2017-11-14

(2)

The aim of this thesis is to explore the use of Bioor­

thogonal Antigens to study the cross-presentation pathway. Bioorthogonal Antigens are antigens carrying bioorthogonal groups in specific amino acid positions within the epitope region that can be reacted selec­

tively within/on the cell using bioorthogonal ligation strategies. Incorporation of bioorthogonal groups into antigens has an advantage over other methods be­

cause most of the groups are stable to proteolysis and are small enough to have a minimal impact on rout­

ing and loading onto MHC-1 molecules. In the future, these antigens have potential to be applied for imaging of the entire cross-presentation pathway using a single bioorthogonal handle.

Bioorthogona I Antigens

Joanna B. Pawlak

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