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Membrane heterogeneity : from lipid domains to curvature effects Semrau, S.

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Membrane heterogeneity : from lipid domains to curvature effects

Semrau, S.

Citation

Semrau, S. (2009, October 29). Membrane heterogeneity : from lipid domains to curvature effects. Casimir PhD Series. Retrieved from https://hdl.handle.net/1887/14266

Version: Not Applicable (or Unknown)

License: Leiden University Non-exclusive license Downloaded from: https://hdl.handle.net/1887/14266

Note: To cite this publication please use the final published version (if applicable).

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Stellingen

behorende bij het proefschrift Membrane heterogeneity

From lipid domains to curvature effects

1. The line tension between liquid ordered and liquid disordered lipid membrane domains can be determined with high accuracy by anal- ysis of shape and fluctuations of fully phase-separated vesicles.

Chapter 2 Semrau et al., Phys. Rev. Lett., 100, 088101 (2008)

2. At a line tension of ≈ 1pN lipid rafts will not exceed a size of 10nm in living cells. Domains of that size can transport only a few proteins, which renders the classical lipid raft concept meaningless.

Chapter 2 Hancock, Nat. Rev. Mol. Cell Biol.,7, 456 (2006)

3. The occurrence of meta-stable liquid ordered domains is due to mem- brane mediated interactions between the domains.

Chapter 3 Baumgart et al., Nature, 425,821 (2003)

4. The preferred length scale found in the distribution of domain sizes is set by the magnitude of the membrane mediated interaction.

Chapter 3 Semrau et al., Biophys. J., 96, 4906 (2009)

5. Membrane mediated interactions between lipid domains lead to spon- taneous sorting according to domain size. A similar effect should also sort proteins which impose a local curvature on the membrane.

Chapter 4

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6. In single-molecule tracking experiments the mean squared displace- ment (and therefore diffusion behavior) of the molecules can be re- trieved without reconstructing individual trajectories.

Chapter 5 Semrau et al., Biophys. J., 92 , 613 (2007)

7. The Adenosine A1 receptor (a G protein coupled receptor) interacts with membrane microdomains upon stimulation.

Chapter 6

8. Decoupling of the Adenosine A1 receptor from its G protein leads to an increased diffusion coefficient of the receptor. The receptor is precoupled to the G protein which mediates the interaction with membrane domains.

Chapter 6

9. Despite its poor photostability YFP (and other fluorescent proteins) can be used to count single molecules in vivo, which enables the quantification of protein clustering

Chapter 7

10. The membrane anchor of H-Ras is found in clusters due to interac- tions with microdomains.

Chapter 7 Lommerse et al., J. Cell Sci., 118, 1799 (2005)

Stefan Semrau October 29, 2009

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