The ATP-sensitive potassium channel in the heart. Functional, electrophysiological and molecular aspects - Titlepage

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The ATP-sensitive potassium channel in the heart. Functional,

electrophysiological and molecular aspects

Remme, C.A.

Publication date

2002

Link to publication

Citation for published version (APA):

Remme, C. A. (2002). The ATP-sensitive potassium channel in the heart. Functional,

electrophysiological and molecular aspects.

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Propositions/Stellingen

' T h e ATP-sensitive potassium channel in the heart: functional,

electrophysiological and molecular aspects"

Carol Ann Remme

/. During myocardial ischemia, cardioprotection conferred by ATP-sensitive potassium

(K

) channel openers may, at least partly, be attributed to a reduction in

noradrenaline release within the myocardium, (this thesis)

2. In anoxic myocytes, sarcolemmal K

channel activation is essential for

postponement of irreversible damage to occur, whereas mitochondrial K

channel

activation by itself has no protective effect, (this thesis)

3. The apparent contradictory results regarding pro- and anti-arrhythmic effects of K

channel openers can be explained by the fact that K

channel openers may increase

the incidence of early, re-entrant (phase la) arrhythmias, but decrease the occurrence

of non-re-entrant (phase lb) arrhythmias, (this thesis)

4. The K

channel subunit Kir6.2 can traffick to the plasma membrane and form

functional, ATP-sensitive potassium channels in the absence of a sulfonylurea

receptor, (this thesis)

5. The K

channel subunit Kiró.1 shows important structural and functional

characteristics different from those of Kir6.2, and may form part of the mitochondrial

K

channel complex, (this thesis)

6. The two-step real-time PCR technique using the LightCycier System provides a

sensitive and specific method for measuring mRNA expression levels of low abundant

genes such as ion channels, (this thesis)

7. Although the K

channel subunit Kir6.1 is highly expressed in both atrial and

ventricular tissue, its functional role in the heart remains to be elucidated, (this thesis)

8. Due to its hair growth stimulating effects, the K

channel opener minoxidil should be

considered a true (pre)conditioner.

9. The most exciting phrase to hear in science, the one that heralds new discoveries, is

not "Eureka!" (I found it!), but "That's funny...". (Isaac Asimov)

10. The great thing about standards is that there are so many.

/ / . T h e art of medicine consists of amusing the patient while nature cures the disease.

(Voltaire)

12. Destiny was invented by people who can't stand the fact that everything that happens

is accidental.

13. When people don't know what you are doing, they don't know what you're doing

w ron 2.

T h e ATP-sensitive potassium channel in the heart

-T h e A-TP-sensitive potassium channel in the heart: functional, electrophysiological and molecular aspects / by Carol Ann Remme / Thesis University of Amsterdam / with summary in Dutch

© 2002 by Carol A n n Remme

N o part of this thesis may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, without prior permission of the author.

ISBN 90-9015598-8 Cover design by the author

T h e ATP-sensitive potassium channel in the heart

functional, electrophysiological and molecular aspects

-A C -A D E M I S C H P R O E F S C H R I F T

ter verkrijging van de graad van doctor aan de Universiteit van Amsterdam op gezag van de Rector Magnificus

prof.mr. P.F. van der Heijden

ten overstaan van een door het college voor promoties ingestelde commissie, in het openbaar te verdedigen in de Aula der Universiteit

op woensdag 27 maart 2002, te 12.00 uur

door

Carol A n n R e m m e geboren te Utrecht

Promotiecommissic:

Promotores: Prof.dr. A.A.M. Wilde Prof.dr. A.F.M. Moorman

Overige leden: Prof.dr. H.J. Jongsma Prof.dr. C.G. Nichols Dr. D J . Duncker Prof.dr. M.J.Janse Dr. M.J.B, van den Hoff

faculteit Geneeskunde

T h e research described in this thesis was conducted at the Laborator}' of Experimental Cardiology (Head: Prof.dr. A.A.M. Wilde) and the Department of Anatomy and Embryology (Head: Prof.dr. A.F.M. Moorman) of the Academie Medical Center, Amsterdam, T h e Netherlands.

T h e study described in this thesis was supported by a grant of the Netherlands Heart Foundation (NHF-96.018).

Financial support by the Netherlands Heart Foundation and the Academic Medical Center for the publication of this thesis is gratefully acknowledged.

Additional financial support for the publication of this thesis was generously provided by B A R D Benelux N.V., Byk Nederland B.V., Guidant Nederland B.V., Sender Nederland B.V., St. Jude Medical B.V., Tramedico B.V., and Yamanouchi Pharma B.V.

U-bas Tout est muf et tout est sauvage

Ubre continent sans grillage let, nos rêves so/it étroits C'est pour ca que j'irai la-bas

Ld-bas Faut du coeur et faut du courage Mais tout est possible a mon age Si tu as la force et lafoi Uor est a portee de tes doigts C'est pour ca que j'irai la-bas

(Jean-Jacques Goldman)