Filling the gaps: The endothelium in regulating vascular leakage and leukocyte extravasation - Front matter

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Filling the gaps

The endothelium in regulating vascular leakage and leukocyte extravasation

Schimmel, L.

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2018

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Citation for published version (APA):

Schimmel, L. (2018). Filling the gaps: The endothelium in regulating vascular leakage and

leukocyte extravasation.

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Filling the gaps

The endothelium in regulating

vascular leakage and leukocyte

extravasation

Lilian Schimmel

Filling the gaps: The endothelium in regulating vascular leakage and leukocyte extravasation Lilian Schimmel

Uitnodiging

voor het bijwonen van de

openbare verdediging van

het proefschrift

Filling the gaps: The

endothelium in regulating

vascular leakage and

leukocyte extravasation

door

Lilian Schimmel

Vrijdag 15 juni 2018

om 14:00 uur

Agnietenkapel

Oudezijds Voorburgwal

231, Amsterdam

Na afloop bent u van

harte uitgenodigd voor de

receptie ter plaatse

Lilian Schimmel

paranimfen

Mark Hoogenboezem

Simon Tol

Filling the gaps

The endothelium in regulating

vascular leakage and leukocyte

extravasation

ISBN: 978-94-6233-962-0

The studies performed in this thesis were performed at the Department of Plasma Proteins, Molecular Cell Biology Lab, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of

Amsterdam, The Netherlands and financially supported by the Rembrandt Institute of Cardiovascular Science.

Printing of this thesis was financially supported by Sanquin Research and Vasculitis Stichting.

Photo cover by Willem Schimmel Layout by Ivar Noordstra

Printed by Gildeprint © 2018 by Lilian Schimmel All rights reserved

Filling the gaps

The endothelium in regulating vascular leakage and leukocyte extravasation

ACADEMISCH PROEFSCHRIFT

ter verkrijging van de graad van doctor aan de Universiteit van Amsterdam op gezag van de Rector Magnificus

prof. dr. ir. K.I.J. Maex

ten overstaan van een door het College voor Promoties ingestelde commissie, in het openbaar te verdedigen in de Agnietenkapel

op vrijdag 15 juni 2018, te 14.00 uur

door

Lilian Schimmel

Promotiecommissie:

Promotor: prof. dr. P.L. Hordijk Universiteit van Amsterdam Copromotores: dr. J.D. van Buul Universiteit van Amsterdam

dr. V. de Waard AMC-UvA

Overige leden: prof. dr. T.W.J. Gadella Universiteit van Amsterdam prof. dr. C.J.M de Vries Universiteit van Amsterdam prof. dr. E. Lutgens Universiteit van Amsterdam dr. J.C. Sluimer Maastricht University

prof. dr. A. Sonnenberg Leiden Academic Centre for

Drug Research

“All we know is still infinitely less than all that remains unknown”

Table of contents

Leukocyte transendothelial migration: A local affair F-actin rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signaling

Endothelial RhoB and RhoC are dispensable for leukocyte diapedesis and for maintaining vascular integrity during diapedesis

The Rho-GEFs FGD5 and Tuba differentially regulate the small GTPase Cdc42 to control leukocyte

extravasation and vascular permeability

Stiffness-induced endothelial DLC-1 expression forces leukocyte spreading through stabilization of the ICAM-1 adhesome

Rac1 activation by the endothelial GEF Tiam1 marks sites for leukocyte diapedesis through guiding junctional membrane ruffles

Summary and concluding remarks Nederlandse samenvatting Curriculum Vitae Portfolio Publication list Dankwoord Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Chapter 8 Addendum