University of Groningen
Multidrug Resistance Regulators as Artificial Enzymes
Gutierrez de Souza, Cora
DOI:
10.33612/diss.168445268
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Publication date: 2021
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Gutierrez de Souza, C. (2021). Multidrug Resistance Regulators as Artificial Enzymes. University of Groningen. https://doi.org/10.33612/diss.168445268
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1. The lack of natural catalytic activity of multidrug resistance regulators and the concomitant lack of evolutionary bias towards a specific reactivity renders them a useful “white canvas” in artificial metalloenzyme design.
2. The versatility of a scaffold can come at expense of efficiency. Advancements in protein engineering will allow to obtain high efficiencies from versatile scaffolds.
3. The appropriate controls at the beginning of a project might show outcomes differing from the expected ones and can shift the direction of the research. (Chapters 4 and 5)
4. The use of rigid scaffolds in enzyme design risks over-design, whereas flexible structures allow the proteins to accommodate ligands in the ideal conformation, sometimes at expenses of predictability. (Chapter 3)
5. Apart from knowledge on the subject and lab-skills, a big influence on the course of a PhD is related to transferable skills (knowing when to ask for help or delegate, problem solving, organization, goal-directed work…) and a bit of luck.
6. Despite recent efforts in the scientific community toward a more inclusive system,
underrepresentation of women and minorities in science continues to be an issue that needs to be tackled in all levels of education and society.
7. Paradoxically, most “last experiments” of a project are not the last experiments.
8. Nature has already found a solution to most scientifically interesting challenges. Hence, natural models are the best source of inspiration.
9. Strengthening science communication is a powerful tool to combat the frightening amount of misinformation of the digital era, to change the old scientists’ stereotypes to a more equalitarian view; and to transfer clear and rigorous information to ensure the impact of science on society.
