Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop development - supporting figure1

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Locus-dependent selection in crop-wild hybrids of lettuce under field conditions

and its implication for GM crop development

Hooftman, D.A.P.; Flavell, A.J.; Jansen, H.; den Nijs, H.C.M.; Syed, N.H.; Sørensen, A.P.; Orozco-ter Wengel, P.; van de Wiel, C.C.M.

DOI

10.1111/j.1752-4571.2011.00188.x

Publication date 2011

Document Version Final published version Published in

Evolutionary Applications

Link to publication

Citation for published version (APA):

Hooftman, D. A. P., Flavell, A. J., Jansen, H., den Nijs, H. C. M., Syed, N. H., Sørensen, A. P., Orozco-ter Wengel, P., & van de Wiel, C. C. M. (2011). Locus-dependent selection in crop-wild hybrids of lettuce under field conditions and its implication for GM crop

development. Evolutionary Applications, 4(5), 648-659. https://doi.org/10.1111/j.1752-4571.2011.00188.x

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0 10 20 30 40 0 0.2 0.4 0.6 0.8

Quasi- P-value derived from simulations

# D a ta p o in ts ( N = 2 1 1 )

Distortion to L. serriola Distortion to L. sativa

1.0

Control

0 25 50 75 100 125 0 0.2 0.4 0.6 0.8

# D a ta p o in ts ( N = 2 2 7 )

1.0

S

₁ 0 10 20 30 40 0 0.2 0.4 0.6 0.8

# D a ta p o in ts ( N = 1 7 1 )

1.0

BC

₁ 0 25 50 75 100 0 0.2 0.4 0.6 0.8

# D a ta p o in ts ( N = 2 1 5 )

1.0

BC

_1S1 0 10 20 30 40 50 < -25 > -21 > -16 > -11 > -6 > -1

P-value derived from Chi-square (logaritmic: 10x₎ # D a ta p o in ts S1 BC1 BC1S1

Figure S1. Histograms of P-value distributions for distortion in the direction of containing

more L. serriola or L. sativa alleles based on a Monte Carlo algorithm, 50,000x drawing N virtual plants from the expected distribution per locus. The observed distribution was compared to these confidence intervals. Each locus was independently drawn per virtual plant. Left-above:

control population grown in the greenhouse, Right-above: S1 generation under field exposure;

Left-middle: BC1 generation under field exposure. Right-middle: BC1S1 generation under field

exposure. Left-below: P-value distribution for all 3 populations for significant alterations in LD

compared to the control generation; note the 10x scaling of x-axis. Chi-square testing was used

for testing of statistical significance, using LD and (1-LD) for both expected (control) and observed (one of the populations) as variables (df = 1).