Complement is activated
during normothermic machine perfusion of porcine and
human discarded kidneys
In preperation
Neeltina M. Jager
Leonie H. Venema
Henri G. D. Leuvenink
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559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager Processed on: 10-5-2021 Processed on: 10-5-2021 Processed on: 10-5-2021
Processed on: 10-5-2021 PDF page: 115PDF page: 115PDF page: 115PDF page: 115
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Time NMP (hours)
ng/ml
C3a in perfusate
**
Time NMP (hours)
Arbitrary units/ml
C5b-9 in perfusate
**
* *
559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager Processed on: 10-5-2021 Processed on: 10-5-2021 Processed on: 10-5-2021
Processed on: 10-5-2021 PDF page: 116PDF page: 116PDF page: 116PDF page: 116
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Time NMP (hours)
pg/ml
IL-6 in perfusate
**
Time NMP (hours)
pg/ml
IL-8 in perfusate
**
* **
**
*
559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager Processed on: 10-5-2021 Processed on: 10-5-2021 Processed on: 10-5-2021
Processed on: 10-5-2021 PDF page: 117PDF page: 117PDF page: 117PDF page: 117
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0 5000 10000 15000 20000 25000 0
IL-6 in perfusate
C3a in perfusate
r = 0.83 p < 0.001
0 2000 4000 6000
0
IL-8 in perfusate
C3a in perfusate r = 0.81
p < 0.001
559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager Processed on: 10-5-2021 Processed on: 10-5-2021 Processed on: 10-5-2021
Processed on: 10-5-2021 PDF page: 118PDF page: 118PDF page: 118PDF page: 118
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Low C5b-9 High C5b-9 0
1 2 3 4
Creatinine clearance (mL/min/100 gr)
Creatinine clearance after 4 hours of NMP
**
559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager Processed on: 10-5-2021 Processed on: 10-5-2021 Processed on: 10-5-2021
Processed on: 10-5-2021 PDF page: 119PDF page: 119PDF page: 119PDF page: 119
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Time NMP (hours)
ng/ml
C3 in perfusate
0 2 4 6
Time NMP (hours)
ng/ml
C3d in perfusate
*
Time NMP (hours)
C3d/C3 ratio
C3d/C3 ratio in perfusate
**
*
559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager Processed on: 10-5-2021 Processed on: 10-5-2021 Processed on: 10-5-2021
Processed on: 10-5-2021 PDF page: 120PDF page: 120PDF page: 120PDF page: 120
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