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  Complement is activated

during normothermic machine perfusion of porcine and

human discarded kidneys

In preperation

Neeltina M. Jager

Leonie H. Venema

Henri G. D. Leuvenink

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559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager Processed on: 10-5-2021 Processed on: 10-5-2021 Processed on: 10-5-2021

Processed on: 10-5-2021 PDF page: 115PDF page: 115PDF page: 115PDF page: 115

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Time NMP (hours)

ng/ml

C3a in perfusate

**

Time NMP (hours)

Arbitrary units/ml

C5b-9 in perfusate

**

* *

 

559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager Processed on: 10-5-2021 Processed on: 10-5-2021 Processed on: 10-5-2021

Processed on: 10-5-2021 PDF page: 116PDF page: 116PDF page: 116PDF page: 116

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Time NMP (hours)

pg/ml

IL-6 in perfusate

**

Time NMP (hours)

pg/ml

IL-8 in perfusate

**

* **

**

*

  

559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager Processed on: 10-5-2021 Processed on: 10-5-2021 Processed on: 10-5-2021

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0 2000 4000 6000

0

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p < 0.001

559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager Processed on: 10-5-2021 Processed on: 10-5-2021 Processed on: 10-5-2021

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Low C5b-9 High C5b-9 0

1 2 3 4

Creatinine clearance (mL/min/100 gr)

Creatinine clearance after 4 hours of NMP

**

559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager Processed on: 10-5-2021 Processed on: 10-5-2021 Processed on: 10-5-2021

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Time NMP (hours)

ng/ml

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0 2 4 6

Time NMP (hours)

ng/ml

C3d in perfusate

*

Time NMP (hours)

C3d/C3 ratio

C3d/C3 ratio in perfusate

**

*

  

559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager 559099-L-sub01-bw-Jager Processed on: 10-5-2021 Processed on: 10-5-2021 Processed on: 10-5-2021

Processed on: 10-5-2021 PDF page: 120PDF page: 120PDF page: 120PDF page: 120

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