Cover Page
The handle http://hdl.handle.net/1887/30141 holds various files of this Leiden University dissertation
Author: Zheng, Tingting Title: Zipping into fusion Issue Date: 2014-12-17
Zipping into Fusion
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van de Rector Magnificus Prof.mr. C.J.J.M. Stolker, Volgens besluit van het College voor Promoties
Te verdedigen op woensdag 17 december 2014 Klokke 12.30 uur
door Tingting Zheng Geboren te China
In 1982
Promotiecommissie
Promotor: Prof. Dr. Ir. J. G. E. M. Fraaije
Co-promotor: Dr. A. Kros
Overige leden: Prof. Dr. M. C. Van Hemert Prof. Dr. J. Lugtenburg Dr. J. A. A. W. Elemans
Contents
1. Introduction 1
2. Probing coiled-coil assembly by paramagnetic NMR spectroscopy 9
3. An antiparallel tetrameric coiled-coil 45
4. Controlled liposome fusion mediated by SNARE protein mimics 83 5. Controlling the rate of coiled coil driven membrane fusion 115
6. Increasing the membrane fusion efficiency by reducing undesired peptide-
peptide interactions 143
Summary 175
Samenvatting 181
Abbreviations 187
Curriculum vitae 191
List of publications 193
Acknowledgements 195