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Cover Page The handle http://hdl.handle.net/1887/30141 holds various files of this Leiden University dissertation Author: Zheng, Tingting Title: Zipping into fusion Issue Date: 2014-12-17

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Cover Page

The handle http://hdl.handle.net/1887/30141 holds various files of this Leiden University dissertation

Author: Zheng, Tingting Title: Zipping into fusion Issue Date: 2014-12-17

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Zipping into Fusion

Proefschrift

ter verkrijging van

de graad van Doctor aan de Universiteit Leiden,

op gezag van de Rector Magnificus Prof.mr. C.J.J.M. Stolker, Volgens besluit van het College voor Promoties

Te verdedigen op woensdag 17 december 2014 Klokke 12.30 uur

door Tingting Zheng Geboren te China

In 1982

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Promotiecommissie

Promotor: Prof. Dr. Ir. J. G. E. M. Fraaije

Co-promotor: Dr. A. Kros

Overige leden: Prof. Dr. M. C. Van Hemert Prof. Dr. J. Lugtenburg Dr. J. A. A. W. Elemans

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Contents

1. Introduction 1

2. Probing coiled-coil assembly by paramagnetic NMR spectroscopy 9

3. An antiparallel tetrameric coiled-coil 45

4. Controlled liposome fusion mediated by SNARE protein mimics 83 5. Controlling the rate of coiled coil driven membrane fusion 115

6. Increasing the membrane fusion efficiency by reducing undesired peptide-

peptide interactions 143

Summary 175

Samenvatting 181

Abbreviations 187

Curriculum vitae 191

List of publications 193

Acknowledgements 195

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