Cover Page The handle http://hdl.handle.net/1887/43990

Cover Page

The handle http://hdl.handle.net/1887/43990 holds various files of this Leiden University dissertation

Author: Hiemstra, Steven

Title: High throughput microscopy of mechanism-based reporters in drug-induced liver injury

Issue Date: 2016-11-09

Cirruculum Vitae

153

Curriculum Vitae

Steven (Willem) Hiemstra was born in Naaldwijk, The Netherlands, on November 29 ^th 1988. He went to the Interconfessional College Westland in Naaldwijk, where he obtained his VWO diploma in 2007, with the major in Natuur en gezondheid. In 2007, he started his study Biology at the University of Utrecht, where he focused on molecular biology and obtained his Bachelor Biology in 2010. In the same year he started his Master “Molecular and Cellular Sciences” at Leiden University. During his Master he studied the role of transposase DAYSLEEPER in Arabidopsis thaliana in the department Molecular and Developmental Genetics under the supervision of dr. Marijn Knip, dr. Sylvia de Pater and prof. Paul Hooykaas.

Directly after completion his Master in June 2012, he started as a PhD student at the Leiden Academic Center for Drug Research at the Leiden University, on the project: “High throughput microscopy of mechanism-based reporters in drug-induced liver injury" as part of the IMI MIP- DILI project. The research was conducted under the supervision of prof. Bob van de Water.

Since July 2016 he is employed as a post-doctoral researcher on the same project.

Appendix

154

List of publications

Quantitative High Content Imaging of Cellular Adaptive Stress Response Pathways in Toxicity for Chemical Safety Assessment.

Steven Wink, Steven Hiemstra, Suzanna Huppelschoten, Erik Danen, Marije Niemeijer, Giel Hendriks, Harry Vrieling, Bram Herpers and Bob van de Water

Chem Res Toxicoly, 2014 Mar 17;27(3):338-55.

PMID: 24450961

DAYSLEEPER: a nuclear and vesicular-localized protein that is expressed in proliferating tissues.

M. Knip, S. Hiemstra, A. Sietsema, M. Castelein, S. de Pater and P.J.J. Hooykaas BMC Plant biology, 2013 dec, 12;13:211.

PMID: 24330683

High Content Imaging-based BAC-GFP Toxicity Pathway Reporters to Assess Chemical Adversity Liabilities.

Steven Wink ^‡ , Steven Hiemstra ^‡ , Bram Herpers and Bob van de Water Archives of Toxicology, 2016 June, (June 2016).

PMID: 27358234

‡ Both authors contributed equally

Automated live cell imaging of adaptive stress responses for assessment of drug-induced liver injury (DILI) liabilities.

Steven Wink ^‡ , Steven Hiemstra ^‡ , Suzanne Huppelschoten ^‡ , Janna E. Klip, Bob van de Water Manuscript in preparation

‡ Authors contributed equally

A 3D HepG2 GFP reporter platform to screen for drug-induced liver injury liability.

Steven Hiemstra, Steven Wink, Karen van den Nieuwendijk, Sreenivasa Ramaiaghari, Anita Dankers, Hans de Bont and Bob van de Water.

Manuscript in preparation

List of publications

155 High content imaging-based comprehensive dynamic landscapes of oxidative stress response and DNA damage signaling during chemical exposure.

Steven Hiemstra ^‡ , Marije Niemeijer ^‡ , Esmee Koedoot, Steven Wink, Janna Klip, Matthijs Vlasveld, Elisabeth de Zeeuw, Bram van Os, Andrew White and Bob van de Water Manuscript in preparation

‡ Both authors contributed equally

Novel regulators of Nrf2 activation relevant for drug-induced liver injury identified by a RNAi- based high throughput microscopy screen.

Steven Hiemstra, Marije Niemeijer, Bram Herpers, Ian Copple, Steven Wink, Jeroen Esselink and Bob van de Water

Manuscript in preparation

A novel Nrf2 liver model reveals critical factors in cell death onset in drug-induced liver injury.

Steven Hiemstra ^‡ , Daniel Kaschek ^‡ , Mirjam Frehling-Kascheck, Isoude Kuijper, Luc Bischoff, Jeroen Esselink, Joost Beltman, Jens Timmer and Bob van de Water

Manuscript in preparation

‡ Both authors contributed equally