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The handle http://hdl.handle.net/1887/62028 holds various files of this Leiden University dissertation.

Author: Zhou, Y.

Title: Exploring novel regulators and enzymes in salicylic acid-mediated plant defense Issue Date: 2018-05-09

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Exploring novel regulators and enzymes in salicylic acid-mediated

plant defense

Yingjie Zhou

周莹洁

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Yingjie Zhou

Exploring novel regulators and enzymes in salicylic acid-mediated plant defense

PhD thesis, Leiden University, 2018

© Yingjie Zhou (2018). All rights reserved. No part of this thesis may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, without the prior written permission of the copyright holder.

Cover designed by Yingjie Zhou

Printed by Ridderprint in the Netherlands ISBN:978-94-6299-956-5

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Exploring novel regulators and enzymes in salicylic acid-mediated

plant defense

Proefschrift

Ter verkrijging van

de graad van Doctor aan de Universiteit Leiden,

op gezag van de Rector Magnificus Prof. mr. C.J.J.M. Stolker, volgens besluit van het College voor Promoties

te verdedigen op woensdag 9 mei 2018 klokke 11:15 uur

door

Yingjie Zhou

Geboren te Chengdu (China) in 1986

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Promotiecommissie

Promotor: Prof. dr. J. Memelink Co-promotor: Dr. H.J.M. Linthorst

Overige leden:

Prof. dr. H.P. Spaink Prof. dr. P.J.J. Hooykaas Prof. dr. R. Offringa

Prof. dr. G.C. Angenent (Wageningen UR, The Netherlands) Dr. M.I. Carqueijero (University of Tours, France)

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Contents

Chapter 1 General introduction 7

Chapter 2 AP/ERF and WRKY transcription factors involved in the coordinated regulation of the salicylic acid signaling pathway in Arabidopsis thaliana

35

Chapter 3 Heterologous expression of Arabidopsis PBS3 generates elevated SA content in E.coli

65

Chapter 4 A genetically engineered E. coli biosensor for screening of cDNA libraries for isochorismate pyruvate lyase-encoding cDNAs

89

Chapter 5 Summary 113

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