Catalytic mechanism and protein engineering of copper-containing
nitrite reductase
Wijma, Hein Jakob
Citation
Wijma, H. J. (2006, February 9). Catalytic mechanism and protein engineering of
copper-containing nitrite reductase. Retrieved from https://hdl.handle.net/1887/4302
Version:
Corrected Publisher’s Version
License:
Licence agreement concerning inclusion of doctoral thesis in the
Institutional Repository of the University of Leiden
Downloaded from:
https://hdl.handle.net/1887/4302
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List of publications
Wijma, H.J., Boulanger, M .J., M olon, A., Fittipaldi, M ., Huber, M ., M urphy, M .E.P., Verbeet, M .P., and Canters, G.W.(2003)Biochemistry 42, 4075-4083, Reconstitution of the Type-1 Active Site of the H145G/A Variants of Nitrite Reductase by Ligand Insertion Astier, Y., Bond, A.M ., Wijma, H.J., Canters, G.W., Hill, H.A.O., and Davis, J.J.(2004) Electroanalysis 16, 1155-1165, A systematic Study of the Influence of Peptide Modification of a Gold Electrode on the Cyclic Voltammetry of Pseudoazurin from Alcaligenes faecalis Strain S-6
Wijma, H.J., Canters, G.W., de Vries, S., and Verbeet, M .P. (2004)Biochemistry 43, 10467-10474, Bidirectional Catalysis by Copper-Containing Nitrite Reductase
Astier, Y., Canters, G.W., Davis, J.J., Hill, H.A.O., Verbeet, M .P., and Wijma, H.J. (2005) Chem. Phys. Chem. 6, 1114-1120, Sensing nitrite through a pseudoazurin-nitrite reductase electron transfer relay
Stirpe, A., Guzzi, R., Wijma, H.J., Verbeet, M .P., Canters, G.W., and Sportelli, L. (2005) Biochim., Biophys. Acta 1752, 47-57, Calorimetric and Spectroscopic Investigations of the Thermal Unfolding of Wild Type and Type-2 Copper Depleted Nitrite Reductase
Fittipaldi, M ., Wijma, H.J., Verbeet, M .P., Canters, G.W., Groenen, E.J.J., and Huber, M . (2005) Biochemistry 44, 15193-15202, The Substrate-Bound Type 2 Copper Site of Nitrite Reductase: The Nitrogen Hyperfine Coupling of Nitrite Revealed by Pulsed EPR
Wijma, H.J., M acPherson, I., Alexandre, M ., Diederix, R.E.M ., Canters, G.W., M urphy, M .E.P., and Verbeet, M .P., submitted to J. M ol. Biol., The Redox-Centre of Copper-Containing Nitrite Reductase Brought under Allosteric Control by Engineering
Wijma, H.J., M acPherson, I., Farver, O., Tocheva, E.I., Pecht, I., Verbeet, M .P., M urphy, M .E.P., and Canters, G.W., to be submitted, Reorganization Energy of the Type-1 Copper Site of Nitrite Reductase Lowered by a Methionine Ligand
Wijma, H.J., Verbeet, M .P. Armstrong, F.A., and Canters, G.W., to be submitted, A Random Sequential Mechanism for Nitrite Binding to Copper-containing Nitrite Reductase Wijma, H.J., Jeuken, L.J.C., Verbeet, M .P., Armstrong, F.A., and Canters, G.W., to be submitted, Protein Film Voltammetry of Copper-Containing Nitrite Reductase Reveals Reversible Inactivation
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Curriculum vitae
Hein Jakob Wijma was born in Drogeham in Fryslân, the Netherlands on the 25th of March 1976. He received his secondary education at the Christelijk Gymnasium in Leeuwarden from 1988 to 1994. He took part as a finalist in both the National Biology Olympiad and the National Chemistry Olympiad.
From 1994 to 1999 he studied Biology at the State University of Groningen. Here he became interested in enzymes, in the alteration of their properties by means of protein engineering, and in the experimental techniques that are used to study enzymes. During his first pre-graduate research project he worked on the regulation of a membrane protein in the group of Prof. Bert Poolman. In his second pre-graduate project he worked on the protein engineering of disulfide bonds under supervision of Dr. Mariël Pikkemaat and Prof. Dick Janssen.
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Acknowledgements
I would like to acknowledge the assistance of the following people during my Ph.D. project. Maxime Alexandre and Lichieh Chen who assisted within the framework of a M.Sc. project. For assistance with experiments in Delft and Oxford Ing. Marc Stampraad and Dr. Kylie Vincent. For carefully proofreading various documents Dr. Sharmini Alagaratnam, Jan Dotinga, Dr. Monica Vlasie, and Dr. Jonathan Worrall. For very valuable discussions Dr. Alexey Cherepanov, Dr. Rutger Diederix, Dr. Dirk Heering, Dr. Michael Machczynski, Dr. Armand Tepper, and Dr. Monica Vlasie. For cooperation in the “scorpion tail” project Dr. Marieke van den Bosch, Dr. Dima Filippov, and Dr. Marc Overhand. Raphael Zwier for support in constructing the equipment used in chapter 4, 5, and 7.
