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The handle http://hdl.handle.net/1887/38868 holds various files of this Leiden University dissertation
Author: Heemskerk, A.A.M.
Title: Exploring the proteome by CE-ESI-MS
Issue Date: 2016-04-28
EXPLORING THE PROTEOME BY CE-ESI-MS
Anthonius Adrianus Maria Heemskerk
Copyright © 2016 Anthonius A.M. Heemskerk. All rights reserved. No part of this thesis may be reproduced in any form or by any means without permission from the author.
Printed by Koninklijke Wöhrmann B.V., Zutphen,The Netherlands
EXPLORING THE PROTEOME BY CE-ESI-MS
PROEFSCHRIFT
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof.mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties te verdedigen op donderdag 28 april 2016
klokke 13.45 uur door
Anthonius Adrianus Maria Heemskerk geboren te Haarlem in 1985
promotoren: prof.dr. A.M. Deelder copromotor: dr. O.A. Mayboroda
Leden promotie commissie: prof. dr. J.W. de Fijter prof. dr. R.E.A.M. Tollenaar
prof. dr. D.J.M. Peters prof. dr. G. Somsen
Department of Chemistry,
Division Analytical Chemistry
at VU Univerity, Amsterdam, The Netherlands dr. J. Schappler
School of Pharmaceutical Sciences, at University of Geneva, Switzerland
Table of Contents
Introduction 7
Chapter 1 21
Ultra-Low flow ESI-MS for improved ionization efficiency in phosphoproteomics
Chapter 2 41
Coupling porous sheathless interface MS with transient-ITP in neutral capillaries for improved sensitivity in glycopeptide analysis
Chapter 3 53
Optimization of capillary electrophoresis-mass spectrometry loadability and separation power
Chapter 4 63
CE-MS for proteomics: advances in interface development and application (2007-2011)
Chapter 5 91
CE-ESI-MS for bottom-up proteomics: advances in separation, interfacing and applications
Chapter 6 121
Workflow for Integrating CE-MS and LC-MS Bottom-up Proteomics Data from SDS-PAGE Pre-fractionated Samples
Chapter 7 137
Proteomics Analysis of laser micro-dissected and sieve isolated Human Glomeruli from frozen tissue by t-ITP-CZE-MS
Discussions and Conclusion 153
Appendices 163
Summary
Nederlandse Samenvatting Dankwoord / Acknowledgments Curriculum Vitae
List of publications
