Cover Page The handle http://hdl.handle.net/1887/87241 h

Cover Page

The handle http://hdl.handle.net/1887/87241 holds various files of this Leiden University dissertation.

Author: Zhang, W.

CE-MS for metabolomics:

advancing performance and

detection sensitivity

CE-MS for metabolomics:

advancing performance and detection sensitivity

Proefschrift

ter verkrijging van

de graad van Doctor aan de Universiteit Leiden,

op gezag van Rector Magnificus Prof. Mr. C.J.J.M. Stolker,

volgens besluit van het college voor Promoties

te verdedigen op dinsdag 31 Maart 2020

klokke 16:15 uur

door

Wei Zhang | 张伟

Promotor

Prof. dr. Thomas Hankemeier

Copromotor

Dr. Rawi Ramautar

Promotiecommissie

Prof. dr. Hubertus Irth (Chair)

Leiden University, the Netherlands

Prof. dr. Joke Bouwstra (Secretary)

Leiden University, the Netherlands

Prof. dr. Erik Danen

Leiden University, the Netherlands

Prof. dr. Philip Britz-McKibbin

McMaster University, Canada

Prof. dr. Govert W. Somsen

Vrije Universiteit Amsterdam, the Netherlands

Prof. dr. Marie-José Goumans

Leiden University Medical Center, the Netherlands

Contents

Chapter 1

General introduction and scope 1

Chapter 2

Next-generation capillary electrophoresis–mass spectrometry approaches in metabolomics

Current opinion in biotechnology (2017) 13

Chapter 3

Part 1 Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic

Profiling of Biological Samples

JoVE (Journal of Visualized Experiments) (2016) 25

Part 2 Capillary Electrophoresis-Mass Spectrometry for Metabolic Profiling of

Biomass-Limited Samples

Methods in Molecular Biology (2019) 37

Chapter 4

Utility of sheathless capillary electrophoresis-mass spectrometry for metabolic profiling of limited sample amounts

Journal of Chromatography B (2019) 47

Chapter 5

Profiling nucleotides in low numbers of mammalian cells by sheathless CE-MS in positive ion mode: circumventing corona discharge

Electrophoresis (2020) 61

Chapter 6

Assessing the suitability of capillary electrophoresis-mass spectrometry for biomarker discovery in plasma-based metabolomics

Electrophoresis (2019) 79

Chapter 7

CE-MS for metabolic profiling of plasma from an acute mouse model for epileptic seizures

In preparation 103

Chapter 8

A quantitative metabolomics approach to the metabolite corona highlights missed opportunities from a protein centric-view of the bio-nano interface

Manuscript submitted 125

Chapter 9

Conclusions and Perspectives 159

Appendix 167