Cover Page
The following handle holds various files of this Leiden University dissertation: http://hdl.handle.net/1887/80841
Author: Shao, S.
Title: Involvement of host and bacterial factors in Agrobacterium-mediated
transformation
Involvement of host and bacterial factors in
Agrobacterium-mediated transformation
Shuai Shao
Shuai Shao
Involvement of host and bacterial factors in Agrobacterium-mediated transformation PhD thesis, Leiden University, The Netherlands, 2019
Shuai Shao was supported by a grant from the China Scholarship Council (No. 201406740044). ISBN: 978-94-6375-648-8
Printed by Ridderprint, The Netherlands
Involvement of host and bacterial factors in
Agrobacterium-mediated transformation
Proefschrift
Ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus prof. mr. C.J.J.M. Stolker,
volgens besluit van het College voor Promoties te verdedigen op donderdag 28 November 2019
klokke 11:15 uur
door
Shuai Shao
Promotor
Prof. Dr. P.J.J. Hooykaas
Co-promotor
Dr. G.P.H. van Heusden
Promotiecommissie
Prof. Dr. G.P. van Wezel Prof. Dr. J. Memelink Prof. Dr. R. Offringa
Table of contents
Chapter 1 9
General introduction
Chapter 2 29
Analysis of the function of ADA2 in double strand break repair and Agrobacterium-mediated transformation of Saccharomyces cerevisiae
Chapter 3 63
Detection of extrachromosomal circular T-DNA formed during Agrobacterium-mediated transformation
Chapter 4 77
Use of the Auxin-induced degron system to study the role of virulence protein VirD2 in the integration of T-DNA into the plant and yeast genome
Chapter 5 95
Complete sequence of the tumor-inducing plasmid pTiChry5 from the hypervirulent
Agrobacterium tumefaciens strain Chry5
Chapter 6 111
Complete sequence of succinamopine Ti-plasmid pTiEU6 reveals its evolutionary relatedness with nopaline-type Ti-plasmids
