University of Groningen
Topography-mediated myofiber formation and endothelial cell sprouting
Almonacid Suarez, A M
DOI:
10.33612/diss.127414004
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Publication date: 2020
Link to publication in University of Groningen/UMCG research database
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Almonacid Suarez, A. M. (2020). Topography-mediated myofiber formation and endothelial cell sprouting. University of Groningen. https://doi.org/10.33612/diss.127414004
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Topography-mediated myofiber formation
and endothelial cell sprouting
Ana Maria Almonacid Suarez
2020
Topography-mediated myofiber formation and endothelial cell sprouting
Illustration and layout: Ana Maria Almonacid Suarez Printed by: Gildeprint
ISBN: 978-94-034-2747-8 (printed version) ISBN: 978-94-034-2748-5 (digital version) Copyright® Ana Maria Almonacid Suarez
Topography-mediated myofiber
formation and endothelial cell
sprouting
PhD thesis
to obtain the degree of PhD at the University of Groningen
on the authority of the Rector Magnificus Prof. C. Wijmenga
and in accordance with the decision by the College of Deans. This thesis will be defended in public on
Monday 22 June 2020 at 11.00 hours by
Ana Maria Almonacid Suarez
born on 15 November 1989Supervisors
Prof. Marco Harmsen Dr. Patrick van Rijn
Assessment committee
Prof. W. (Wolfgang) Wagner Prof. B.M. (Barbara) Bakker Prof. F. (Floris) Foijer
Paranimfen
Vera Carniello Marloes Sol
Table of Contents
Chapter 1: Introduction and outline of the thesis ...11
1.1. Tissue engineering of skeletal muscle ... 12
1.2. Outline and aim of the thesis ... 20
Chapter 2: Directional topography gradients drive optimum alignment and differentiation of human myoblasts. ...29
INTRODUCTION ... 31
MATERIALS AND METHODS ... 32
RESULTS ... 36
DISCUSSION ... 43
CONCLUSIONS ... 45
SUPPLEMENTARY INFORMATION ... 51
Chapter 3: Topography-driven alterations in endothelial cell phenotype and contact guidance ...53
INTRODUCTION ... 55
MATERIALS AND METHODS ... 56
RESULTS ... 58
DISCUSSION ... 73
CONCLUSION ... 75
SUPPLEMENTARY INFORMATION ... 79
Chapter 4: Topography-mediated myotube and endothelial alignment, differentiation, and extracellular matrix organization for skeletal muscle engineering ...83
INTRODUCTION ... 85
MATERIALS AND METHODS ... 86
RESULTS ... 91
DISCUSSION ... 105
CONCLUSIONS ... 107
Chapter 5: General discussion ...125 Future perspectives ... 131 Concluding remarks ... 132 Summary ...137 Samenvatting ...143 Acknowledgements ...149
