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pGEM-cPSY1 The 1317 bp full-length VvPSY1 was PCR-amplified from cDNA (using the primers VvPSY5’-ATG and VvPSY3’-STOP) and cloned into pGEM

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CONSTRUCT DESCRIPTION

pGEM-cPSY1 The 1317 bp full-length VvPSY1 was PCR-amplified from cDNA (using the primers VvPSY5’-ATG and VvPSY3’-STOP) and cloned into pGEM

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-T Easy

pGEM-cPDS1 The 1749 bp full-length VvPDS1 was PCR-amplified from cDNA (using the primers VvPDS5’-ATG and VvPDS3’-STOP) and cloned into pGEM

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-T Easy

pGEM-cZDS1 The 1752 bp full-length VvZDS1 was PCR-amplified from cDNA (using the primers VvZDS5’-ATG and VvZDS3’-STOP) and cloned into pGEM

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-T Easy

pGEM-cLECY1 The 1593 bp full-length VvLECY1 was PCR-amplified from cDNA (using the primers VvLECY5’-ATG and VvLECY3’-STOP) and cloned into pGEM

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-T Easy

pGEM-cLUT1 The 1692 bp full-length VvECH1 was PCR-amplified from cDNA (using the primers VvECH5’-ATG and VvECH3’-STOP) and cloned into pGEM

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-T Easy

pGEM-cLBCY1 The 1494 bp full-length VvLBCY1 was PCR-amplified from cDNA (using the primers VvLBCY15’-ATG and VvLBCY13’- STOP) and cloned into pGEM

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-T Easy

pGEM-cLBCY2 The full-length 1515 bp VvLBCY2 was PCR-amplified from cDNA (using the primers VvLBCY25’-ATG and VvLBCY23’- STOP) and cloned into pGEM

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-T Easy

pGEM-cBCH1 The 900 bp full-length VvBCH1 was PCR-amplified from cDNA (using the primers VvBCH5’-ATG and VvBCH3’-STOP) and cloned into pGEM

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-T Easy

pGEM-cZEP1 The 1977 bp full-length VvZEP1 was PCR-amplified from cDNA (using the primers VvZEP5’-ATG and VvZEP3’-STOP) and cloned into pGEM

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-T Easy

pGEM-cVDE1 The 1440 bp full-length VvVDE1 was PCR-amplified from cDNA (using the primers VvVDE5’-ATG and VvVDE3’-STOP) and cloned into pGEM

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-T Easy

pGEM-cNCED3 The 1833 bp full-length VvNCED3 was PCR-amplified from cDNA (using the primers VvNCED5’-ATG and VvNCED3’-

STOP) and cloned into pGEM

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-T Easy

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VvPSY1_5’-ATG ATGTCTGTTGCTCTGTTGTGGATTG 60 Amplifies the full-length phytoene synthase 1 encoding gene (VvPSY1) from cDNA (1317 bp). VvPSY1_3’-STOP