University of Groningen
siRNA in precision-cut lung slices: knocking down fibrosis?
Ruigrok, Mitchel
DOI:
10.33612/diss.102801030
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Publication date: 2019
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Ruigrok, M. (2019). siRNA in precision-cut lung slices: knocking down fibrosis?. Rijksuniversiteit Groningen. https://doi.org/10.33612/diss.102801030
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siRNA IN PRECISION-CUT LUNG SLICES:
KNOCKING DOWN FIBROSIS?
Paranymphs
Drs. Carin Biel Drs. Louise van Wijk
COLOPHON
ISBN (printed version): 978-94-034-2130-8 ISBN (digital version): 978-94-034-2129-2
Layout and design by Vera van Ommeren, persoonlijkproefschrift.nl. Printed by Ipskamp Printing, proefschriften.net.
Research presented in this thesis was performed at the department of Pharmaceutical Technology and Biopharmacy of the University of Groningen. Printing of the thesis was supported by the University of Groningen Library and Faculty of Science and Engineering.
Copyright © 2019, Mitchel Ruigrok. All rights reserved. No parts of this publication may be reproduced, transmitted, or translated in any form or by any means, electronic or mechanical, including photocopying, recording or otherwise, without prior permission by the author.
siRNA in precision-cut lung slices: knocking down fibrosis?
Proefschrift
ter verkrijging van de graad van doctor aan de Rijksuniversiteit Groningen
op gezag van de
rector magnificus prof. dr. C. Wijmenga en volgens besluit van het College voor Promoties.
De openbare verdediging zal plaatsvinden op vrijdag 20 december 2019 om 14.30 uur
door
Mitchel Johannes Robertus Ruigrok
geboren op 15 mei 1992 te Leiden
Promotores
Prof. dr. H.W. Frijlink Prof. dr. P. OlingaCopromotor
Dr. W.L.J. HinrichsBeoordelingscommissie
Prof. dr. R. Gosens Prof. dr. W. Jiskoot Prof. dr. R.M. SchiffelersTABLE OF CONTENTS
CHAPTER 1 General introduction
CHAPTER 2 Pulmonary administration of small interfering
RNA: the route to go?
CHAPTER 3 siRNA-mediated RNA interference in
precision-cut tissue slices prepared from mouse lung and kidney
CHAPTER 4 siRNA-mediated protein knockdown in
precision-cut lung slices
CHAPTER 5 Effect of oxygen concentration on cell death,
anti-oxidant transcription, acute inflammation, and cell proliferation in precision-cut lung slices
CHAPTER 6 Knockdown of HSP47 and its effect on fibrogenic
precision-cut lung slices
CHAPTER 7 General discussion and perspectives APPENDIX 1 Summary
APPENDIX 2 Samenvatting APPENDIX 3 Dankwoord
APPENDIX 4 Biography & list of publications
7 15 45 65 87 115 139 154 158 162 166
