University of Groningen
Enhancing the antimicrobial potential of lanthipeptides by employing different engineering
strategies
Zhao, Xinghong
DOI:
10.33612/diss.127409437
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Publication date: 2020
Link to publication in University of Groningen/UMCG research database
Citation for published version (APA):
Zhao, X. (2020). Enhancing the antimicrobial potential of lanthipeptides by employing different engineering strategies. University of Groningen. https://doi.org/10.33612/diss.127409437
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Enhancing the antimicrobial
potential of lanthipeptides by
employing different
engineering strategies
Enhancing the antimicrobial potential of lanthipeptides by
employing different engineering strategies
The scientific studies presented in this thesis were performed at the Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, 9747 AG, The Netherlands.
The candidate was financially supported by the China Scholarship Council (CSC) and the University of Groningen.
Printing of this thesis was financially supported by the Graduate School of Science and Engineering and the University of Grongningen.
Printed by: IPSKAMP Printing
Cover design: Xinghong Zhao
Copyright 2020 Xinghong Zhao
ISBN (printed): 978-94-034-2783-6
Enhancing the antimicrobial
potential of lanthipeptides by
employing different
engineering strategies
PhD thesis
to obtain the degree of PhD at the
University of Groningen
on the authority of the
Rector Magnificus Prof. C. Wijmenga
and in accordance with
the decision by the College of Deans.
This thesis will be defended in public on
Monday 22 June 2020 at 9.00 hours
by
Xinghong Zhao
born on 3 September 1990
in Sichuan, China
Supervisors
Prof. Oscar P. Kuipers Prof. Gert N. MollAssessment Committee
Prof. A.J.M. DriessenProf. G.J. Poelarends Prof. G.P. Van Wezel
Contents
Chapter 1 General introduction 7
Chapter 2 An engineered double lipid II binding motifs-containing lantibiotic displays potent and selective antimicrobial activity against E. faecium
29
Chapter 3 Mimicry of non-ribosomally produced antimicrobials by ribosomal synthesis and posttranslational modification
59
Chapter 4 High-throughput screening for substrate specificity-adapted mutants of the nisin dehydratase NisB
83
Chapter 5 Silver@nisin nanoclusters are highly effective against biofilm-forming pathogens found in wound infection
121
Chapter 6 Summary and General Discussion 139
Samenvatting 151
Acknowledgements 157
