Citation
Vries, F. A. T. de. (2007, January 17). The role of homologous recombination in mitotic and
meiotic double-strand break repair. Retrieved from https://hdl.handle.net/1887/8784
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the
Institutional Repository of the University of Leiden
Downloaded from: https://hdl.handle.net/1887/8784
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The role of homologous recombination in
mitotic and meiotic double- strand break
repair
The role of homologous recombination in
mitotic and meiotic double-strand break
repair
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van de Rector Magnif icus Dr. D. D. Breimer,
hoogl eraar in de f acul teit der Wiskunde en
Natuurwetenschappen en die der Geneeskunde,
vol gens besl uit van het Col l ege voor Promoties
te verdedigen op woensdag 17 januari 2007
kl okke 16. 15 uur
door
Femke Adriana Theodora de Vries
Geboren te Nijmegen,
in 1977
Overige leden: Prof. Dr. L.H.F. Mullenders
Prof. Dr. A.K. Raap
The role of homologous recombination in mitotic and meiotic double-
strand break repair.
ISBN- 10: 90- 9021192- 6
ISBN- 13: 978- 90- 9021192- 3
Printed by Gildeprint Drukkerijen B.V., Enschede
The printing of this thesis was financially supported by:
Makelaarskantoor Rijnland B.V.
Credo
Je mist meer
Dan je meemaakt
Helemaal
Niet erg
© Martin Bril
Contents
Chapter 1 1.1 1.2 1.3
1.4
1.5
1.6 Chapter 2
Chapter 3
Chapter 4
Chapter 5
General Introduction Introduction
DNA double-strand break repair Homologous recombination
1.3.1 Mitotic recombinational repair proteins 1.3.2 Mre11, Rad50 and Nbs1/Xrs2
1.3.3 Rad51 and Rad51 paralogs 1.3.4 Rad52
1.3.5 Rad54
1.3.6 Other proteins involved in HR Recombination during meiosis 1.4.1 Meiosis
1.4.2 Meiotic recombination
1.4.3 Meiotic recombinational repair proteins Protein modification
1.5.1 Ubiquitin and other modifiers 1.5.2 SUMO
1.5.3 SUMO modification of DSB-repair proteins Outline of the thesis
Schizosaccharomyces pombe Rad22A and Rad22B have similar biochemical properties and form multimeric structures.
Inactivation of RAD52 aggravates RAD54 defects in mice but not in Schizosaccharomyces pombe.
The role of SUMO modification in repair of DNA double- strand breaks in D. melanogaster and S. pombe.
Mouse Sycp1 functions in synaptonemal complex assembly, meiotic recombination, and XY body formation.
Summary Samenvatting Abbreviations Curriculum vitae
Appendix: Selected coloured figures
9 11 11 14 17 17 20 21 23 24 26 26 27 29 31 31 32 34 37 61
81
101
123
151 159 167 169 171
