Human embryonic stem cells : advancing biology and cardiogenesis towards functional applications l
Braam, S.R.
Citation
Braam, S. R. (2010, April 28). Human embryonic stem cells : advancing biology and cardiogenesis towards functional applications l. Retrieved from https://hdl.handle.net/1887/15337
Version: Corrected Publisher’s Version
License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden
Downloaded from: https://hdl.handle.net/1887/15337
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stefan r. braam
Human embryonic stem cells
Advancing biology and cardiogenesis towards functional applications
Stefan Robbert Braam
Thesis Leiden University Medical Center
Cover illustration:
Connecting the dots, advancing human embryonic stem cell biology and cardiogenesis towards functional applications
Copyright 2010, Stefan R. Braam, Leiden, The Netherlands. All rights reserved. No part of this book may be reproduced or transmitted, in any form or by any means, without written permission of the author.
Graphic design: Jort Braam / www.studiokern.nl Printed by Digital Printing Partners, Houten ISBN 978-90-9025308-4
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proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden, op gezag van Rector Magnificus Prof. Mr. P.F van der Heijden,
volgens besluit van het College voor Promoties te verdedigen op woensdag 28 April 2010
klokke 15.00 uur
door
stefan robbert braam
geboren te Dalfsen in 1983
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promotor
Prof. Dr. C.L. Mummeryco-promotor
Dr. R. Passieroverige leden
Prof. Dr. W.E. Fibbe Prof. Dr. G.J. van OmmenProf. Dr. H.J. Tanke
Prof. Dr. H.T. Timmers (UMC Utrecht)
The work presented in this thesis was carried out at the Hubrecht Institute (Utrecht) and the department of Anatomy & Embryology Leiden University Medical Center and was supported by a grant from the Dutch Program for Tissue Engineering.
Financial support by the Netherlands Heart Foundation and the J.E Jurriaanse stichting for the publication of this thesis is gratefully acknowledged.
Additional financial support was granted by Becton Dickinson, Boehringer Ingelheim BV and Multichannel Systems.
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chapter 1 6
General Introduction
chapter 2 14
Improved genetic manipulation of human embryonic stem cells
chapter 3 24
Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic manipulation
chapter 4 44
Feeder-free human embryonic monolayer cultures express an epithelial plasma membrane protein profile
chapter 5 54
Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self renewal via αVβ5 integrin
chapter 6 74
Phosphorylation dynamics during early differentiation of human embryonic stem cells
chapter 7 96
Multipotent NKX2-5+ cardiac progenitors derived from human embryonic stem cells
chapter 8 112
Predictive drug induced cardiotoxicity using human embryonic stem cell-derived cardiomyocytes
chapter 9 128
Cardiomyocytes from pluripotent stem cells in regenerative medicine and drug discovery
chapter 10 139
General discussion
