Imaging the translocations of CLIC4 and Epac1 Ponsioen, B.

Considering that ZO-1 has three distinct PDZ do- mains, we examined which (if any) PDZ domain binds PLC β3. We expressed GFP-tagged versions of the three individual PDZ

The most promising of these FRET pairs were subse- quently tested in the Epac sensor. Which of these constructs is recommendable as cAMP sensor in FLIM and ratiometric

To compare the efficiency of 007-AM and 007 in ac- tivating Epac1 in vivo, an Epac1-based fluorescence res- onance energy transfer (FRET) probe was used. In this assay,

In this thesis, we studied the translocations of two proteins: that of chloride intracellular channel 4 (CLIC4) and that of ex- change protein directly activated by cAMP

M.Vliem*, B.Ponsioen*, F.Schwede, W.J.Pannekoek, J.Riedl, M.R.Kooistra, K.Jalink, H.G.Genieser, J.L.Bos and H.Rehmann.. 8-pCPT-2’-O-Me-cAMP-AM: an improved

Epac1 translokeert naar de plasmamembraan na binding van cAMP alsmede door interactie met ERM eiwitten; beide translocaties dragen bij aan efficiënte koppeling naar

Peripheral blood cells were stained with HLA-A2.1 tetramers containing the tyrosinase368–376 peptide followed by staining with a panel of lineage antibodies, as described in

Blades and blade fragments seem to have been especially used for longitudinal motions, mainly on plant material (7/12). Flake and flake fragments are used in different motions on