Identification and characterization of starch and inulin modifying network of Aspergillus niger by functional genomics

Citation

Yuan, X. L. (2008, January 23). Identification and characterization of starch and inulin modifying network of Aspergillus niger by functional genomics.

Institute of Biology Leiden (IBL), Group of Molecular Microbiology, Faculty of Science, Leiden University. Retrieved from

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Identification and Characterization of Starch and Inulin Modifying Network of Aspergillus

niger by Functional Genomics

Xiao-Lian Yuan

Identification and Characterization of Starch and Inulin Modifying Network of Aspergillus

niger by Functional Genomics

Proefschrift





terverkrijgingvan

degraadvanDoctoraandeUniversiteitLeiden,

opgezagvanRectorMagnificusprof.mr.P.F.vanderHeijden,

volgensbesluitvanhetCollegevoorPromoties

teverdedigenop23januari2008

klokke13:45uur

door



XiaoLianYuan



geborenteFeixiAnhui,China

in1974

































Cover:

inraffinose

 DesignedbyXiaoLianYuanandPatrickVermeij

Layout: PatrickVermeij

PrintedBy: PrintpartnersIpskampB.V.Enschede

ISBN: 9789090227085





































Formyparents,

PatrickandJason



⤂㒭៥ⱘ⠊↡

⠅Ҏ੠ܓᄤ













Contents

Chapter1 GeneralIntroduction 9

Chapter2 Construction of inulin and starch specific Aspergillus niger cDNA

expressionlibraries

25

Chapter3 Aspergillusnigergenomewideanalysisrevealsalargenumberofnovel alphaglucanactingenzymeswithunexpectedexpressionprofiles

39

Chapter4 Characterizationoftwonovel,putativelycellwallassociatedandGPI

anchored,glucanotransferaseenzymesofAspergillusniger

65

Chapter5 Databaseminingandtranscriptionalanalysisofgenesencodinginulin modifyingenzymesofAspergillusniger

85

Chapter6 Molecular and biochemical characterization of a novel intracellular invertasefromAspergillusnigerwithtransfructosylatingactivity

105

Chapter7 Identification of InuR, a new Zn(II)2Cys(6) transcriptional activator involvedintheregulationofinulinolyticgenesinAspergillusniger

119

Chapter8 A novel screening method for isolation of mutants involved in inulin signallinginAspergillusniger

143

Summary 159

Samenvatting 163

ᨬ㽕 167

References 171

Publications 185

CurriculimVitae 187

Acknowledgements 189



8