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Force generation in dividing E. coli cells: A handles-on approach using optical tweezers

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Citation

Verhoeven, G. S. (2008, December 2). Force generation in dividing E. coli cells: A handles- on approach using optical tweezers. Retrieved from https://hdl.handle.net/1887/13301

Version: Corrected Publisher’s Version

License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden

Downloaded from: https://hdl.handle.net/1887/13301

Note: To cite this publication please use the final published version (if applicable).

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Force generation in dividing E. coli cells:

A handles-on approach using optical tweezers

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Force generation in dividing E. coli cells:

A handles-on approach using optical tweezers

PROEFSCHRIFT

TER VERKRIJGING VAN DE GRAAD VAN DOCTOR AAN DE UNIVERSITEIT LEIDEN, OP GEZAG VAN DE RECTOR MAGNIFICUS

PROF.MR. P.F. VAN DER HEIJDEN,

VOLGENS BESLUIT VAN HET COLLEGE VOOR PROMOTIES TE VERDEDIGEN OP DINSDAG 2 DECEMBER 2008

TE KLOKKE 11.15 UUR

DOOR

G ERTJAN S EBASTIAAN V ERHOEVEN

GEBOREN TE LEIDERDORP IN 1977

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Referent: Prof. dr. A.J.M. Driessen (Universiteit Groningen) Overige leden: Prof. dr. J.P.M. Tommassen (Universiteit Utrecht)

Dr. L.B. Oddershede (Niels Bohr Institute, Copenhagen)

Prof. dr. H. Schiessel

Prof. dr. T. Schmidt

Prof. dr. J.M. van Ruitenbeek

Force generation in dividing E. coli cells:

A handles-on approach using optical tweezers

©2008 by Gertjan Sebastiaan Verhoeven. All rights reserved.

Nederlandse titel: Krachtgeneratie in delende E. coli cellen: handvatten voor een benadering met een optisch pincet

The work described in this thesis was performed at the FOM Institute for Atomic and Molecular Physics (AMOLF), Kruislaan 407, 1098 SJ Amsterdam as well as at the Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 316, 1098 SM Amsterdam. This work is financially supported by the "Nederlandse organisatie voor Wetenschappelijke Onderzoek (NWO)" as part of the “From Molecule to Cell” program.

ISBN 978-90-77209-28-8

A digital version of this thesis can be downloaded from http://ub.leidenuniv.nl. Printed copies can be obtained by addressing the library at the FOM institute for Atomic and Molecular Physics (AMOLF): library@amolf.nl; Kruislaan 407, 1092 SJ, Amsterdam, The Netherlands.

Printed in the Netherlands by Ponsen & Looijen BV graphical company, Wageningen.

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Aan mijn ouders

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to be resubmitted

Gertjan S. Verhoeven, Marileen Dogterom and Tanneke den Blaauwen. Outer membrane assembly of N- and C-terminal fusions to the OmpA transmembrane domain, to be submitted

Gertjan S. Verhoeven, Tanneke den Blaauwen and Marileen Dogterom. Force-extension curves of DNA tethers attached to outer membrane protein OmpA in a living bacterium, to be submitted

Other articles:

Svetlana Alexeeva, Theodorus W.J. Gadella Jr, Gertjan S. Verhoeven, and Tanneke den Blaauwen. Spectral FRET with Background Unmixing Allows Determination of E. coli Protein Interactions at Native Expression Levels, to be resubmitted

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