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The handle http://hdl.handle.net/1887/57991 holds various files of this Leiden University dissertation
Author: Rossius, S.G.H.
Title: Q-wires': Synthesis, electrochemical properties and their application in electro- enzymology
Issue Date: 2017-09-26
APPENDICES
138
Appendix 1 (A) Cyclic voltammograms of a heptanethiol-modified electrode at different potential ranges in 50 mM MOPS pH 7.2, 5 mM EDTA, 50 mM succinate, 20°C; scan rate: 10 mV/s (B) Voltammograms after addition of E. coli succinate dehydrogenase (black solid line; before addition: gray line); inhibited signal after addition of oxaloacetic acid (40 mM; black dashed line) (C) ‘Catalytic’ current (solid minus dashed black line in B)
A
-30 -20 -10 0 10 20 30
-200 -100 0 100 200 300 400 500
Current (nA)
Potential (mV vs NHE)
B
-20 -10 0 10 20 30
100 150 200 250 300 350 400 450 500
Current (nA)
Potential (mV vs NHE)
C
-0.5 0 0.5 1 1.5 2
100 200 300 400 500
Catalytic current (nA)
Potential (mV vs NHE)
139
Appendix 2 (A) Cyclic voltammograms of a U0- and heptanethiol-modified electrode at different potential ranges in 50 mM MOPS pH 7.2, 5 mM EDTA, 50 mM succinate, 20°C; scan rate: 10 mV/s (B) Catalytic voltammogram after addition of E. coli succinate dehydrogenase (black solid line; before addition: gray line);
inhibited signal after addition of oxaloacetic acid (40 mM; black dashed line) (C) Catalytic current (solid minus dashed black line in B) and its derivative (gray line)
-60 -40 -20 0 20
-200 -100 0 100 200 300 400 500
Current (nA)
Potential (mV vs NHE)
B
-10 -5 0 5 10 15 20 25
100 150 200 250 300 350 400 450 500 550
Current (nA)
Potential (mV vs NHE)
C
-0.3 -0.2 -0.1 0 0.1 0.2 0.3
0 1 2 3 4 5
100 200 300 400 500
Derivative (nA/s)
Catalytic current (nA)
Potential (mV vs NHE)
140
Appendix 3 (A) Cyclic voltammograms of a U1- and heptanethiol-modified electrode at different potential ranges in 50 mM MOPS pH 7.2, 5 mM EDTA, 50 mM succinate, 20°C; scan rate: 10 mV/s (B) Catalytic voltammogram after addition of E. coli succinate dehydrogenase (black solid line; before addition: gray line);
inhibited signal after addition of oxaloacetic acid (40 mM; black dashed line) (C) Catalytic current (solid minus dashed black line in B) and its derivative (gray line)
A
-80 -60 -40 -20 0 20 40 60
-200 -100 0 100 200 300 400 500
Current (nA)
Potential (mV vs NHE)
B
-20 -10 0 10 20
100 150 200 250 300 350 400 450 500 550
Current (nA)
Potential (mV vs NHE)
C
-0.3 -0.2 -0.1 0 0.1 0.2 0.3
0 1 2 3 4 5
100 200 300 400 500
Derivative (nA/s)
Catalytic current (nA)
Potential (mV vs NHE)
141
Appendix 4 (A) Cyclic voltammograms of a U3- and heptanethiol-modified electrode at different potential ranges in 50 mM MOPS pH 7.2, 5 mM EDTA, 50 mM succinate, 20°C; scan rate: 10 mV/s (B) Catalytic voltammogram after addition of E. coli succinate dehydrogenase (black solid line; before addition: gray line);
inhibited signal after addition of oxaloacetic acid (40 mM; black dashed line) (C) Catalytic current (solid minus dashed black line in B) and its derivative (gray line)
-30 -20 -10 0 10 20
-200 -100 0 100 200 300 400 500
Current (nA)
Potential (mV vs NHE)
B
-10 -5 0 5 10 15 20 25
100 150 200 250 300 350 400 450 500
Current (nA)
Potential (mV vs NHE)
C
-0.3 -0.2 -0.1 0 0.1 0.2 0.3
-1 0 1 2 3 4 5 6 7
100 200 300 400 500
Derivative (nA/s)
Catalytic current (nA)
Potential (mV vs NHE)
142
Appendix 5 (A) Cyclic voltammograms of a USAT- and heptanethiol-modified electrode at different potential ranges in 50 mM MOPS pH 7.2, 5 mM EDTA, 50 mM succinate, 20°C; scan rate: 10 mV/s (B) Catalytic voltammogram after addition of E. coli succinate dehydrogenase (black solid line; before addition: gray line);
inhibited signal after addition of oxaloacetic acid (40 mM; black dashed line) (C) Catalytic current (solid minus dashed black line in B) and its derivative (gray line)
A
-50 -40 -30 -20 -10 0 10 20
-200 -100 0 100 200 300 400 500
Current (nA)
Potential (mV vs NHE)
B
-15 -10 -5 0 5 10 15 20 25
100 150 200 250 300 350 400 450 500
Current (nA)
Potential (mV vs NHE)
C
-0.3 -0.2 -0.1 0 0.1 0.2 0.3
-1 0 1 2 3 4 5 6
100 200 300 400 500
Derivative (nA/s)
Catalytic current (nA)
Potential (mV vs NHE)
143
Appendix 6 (A) Catalytic voltammograms of E. coli fumarate reductase (enriched membrane suspension) before (solid line) and after inhibition with ZnSO4 (16 mM;
dashed line); buffer: 50 mM MOPS pH 7.2, 5 mM EDTA, 100 mM fumarate, 20°C;
scan rate: 2 mV/s; electrode modifications: M3 and mercaptohexanol (B) Catalytic current (solid minus dashed line in A) and its derivative (gray line)
-25 -20 -15 -10 -5 0
-350 -300 -250 -200 -150 -100 -50 0
Current (nA)
Potential (mV vs NHE)
B
-0.1 -0.05 0 0.05
-3 -2 -1 0 1
-300 -250 -200 -150 -100 -50
Derivative (nA/s)
Catalytic current (nA)
Potential (mV vs NHE)
144
Appendix 7 (A) Catalytic voltammograms of E. coli cytochrome bo3 (purified) before (solid black line) and after inhibition with KCN (100 µM; dashed line); gray line: before addition of enzyme; buffer: 50 mM MOPS pH 7.2, 5 mM EDTA, lauryl maltoside, 20°C; scan rate: 5 mV/s; electrode modifications: USAT and heptanethiol (B) Catalytic current (solid black line minus dashed line in A) and its derivative (gray line)
A
-500 -400 -300 -200 -100 0 100
-400 -300 -200 -100 0 100 200 300
Current (nA)
Potential (mV vs NHE)
B
-3 -2 -1 0 1 2 3
-150 -120 -90 -60 -30 0
-350 -250 -150 -50 50 150 250
Derivative (nA/s)
Catalytic current (nA)
Potential (mV vs NHE)
