Arterivirus replicase processing : regulatory cascade or Gordian knot? Aken, A.T. van

1 Faculty of Veterinary Medicine, Infectious Diseases and Immunology, Utrecht, the Netherlands, 2 University Medical Centre Utrecht, Eijkman-Winkler Intitute, Utrecht, the

1 Erasmus Medical Centre, Medical Microbiology and Infections Diseases, Rotterdam, the Netherlands, 2 Children’s Hospital of Buffalo, Division of Infectious Diseases, New York,

In line with the previous observations that nsp2 interacts with nsp3 (and nsp3- containing proteins) and could be a cofactor of nsp4-mediated cleavage (Wassenaar et al., 1997),

However, our in vivo assessment of the importance of nsp4-mediated processing of the nsp3-8 region showed that mutants in which cleavage of one of the six sites was blocked are

Crystal structure of nonstructural protein 10 from the severe acute respiratory syndrome coronavirus reveals a novel fold with two zinc-binding motifs.. Emergence of a

The proteolytic activity of recombinant His-tagged nsp4 and both cleaved and uncleaved MBP- nsp4 was demonstrated using a synthetic peptide-based trans cleavage assay, as well as a

Twee grote open leesramen (ORFs) in het 5’-proximale gedeelte van het EAV genoom, ORF1a en ORF1b, coderen voor de replicase polyproteïnen pp1a (1.728 aminozuren) en pp1ab

3 De waarneming dat de mutagenese van elk van de nsp4 klievingsplaatsen in pp1a leidt tot afname of verlies van virale RNA synthese, geeft aan dat maturatie van de