University of Groningen
Robust monooxygenase biocatalysts
Fürst, Maximilian
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Publication date: 2019
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Fürst, M. (2019). Robust monooxygenase biocatalysts: discovery and engineering by computational design. University of Groningen.
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C
URRICULUM
V
ITAE
Maximilian (Max) Fürst was born on 10 August 1988 in Neumarkt in the
German state of Bavaria, where he also grew up and went to high school. In
2007, he moved to Regensburg to perform his alternative civilian service in a
hospital, where he worked in the surgical nursing team.
For his undergrad studies, he moved to Munich, where he obtained a Bachelor
degree in biology at the Ludwig Maximilian University (LMU) in 2011. He
continued at the LMU to obtain a master’s degree in biology in 2013, during
which he spent one semester in Málaga, Spain, as part of the European student
exchange program Erasmus. His master thesis was conducted in the lab of the
chair of the microbiology department, Prof. Dr. Kirsten Jung where he was
working under the supervision of PD Dr. Jürgen Lassak. In the course of this
work, a glycolization strategy was discovered, which bacteria can employ to
activate the universal translation elongation factor P.
Max decided to escape the western society for some time and spent 2014 as a
backpacking gap year, travelling mostly in India, but also in Sri Lanka, Nepal,
Thailand and Myanmar.
Upon his return, he moved to Groningen in the Netherlands to pursue
postgraduate research in biochemistry. Performed in the group of Prof. Dr.
Marco Fraaije, his PhD thesis was part of the EU’s Horizon2020-funded ROBOX
project and focused on the discovery and protein engineering of
monooxygenases. With the aim of prepping these enzymes for biocatalytic
applications, the thesis combined a variety of experimental and computational
techniques.
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IST OF
P
UBLICATIONS
(1) Lassak, J; Keilhauer, EC; Fürst, MJLJ; Wuichet, K; Gödeke, J; Starosta, AL; Chen, J-M; Søgaard-Andersen, L; Rohr, J; Wilson, DN; Häussler, S; Mann, M; Jung, K. Arginine-Rhamnosylation as New Strategy to Activate Translation Elongation Factor P. Nat. Chem. Biol. 2015 (11) 266.
(2) Fürst, MJLJ; Savino, S; Dudek, HM; Gomez Castellanos, JR; Gutierrez de Souza, C; Rovida, S; Fraaije, MW; Mattevi, A. Polycyclic Ketone Monooxygenase from the Thermophilic Fungus Thermothelomyces Thermophila: A Structurally Distinct Biocatalyst for Bulky Substrates. J. Am. Chem. Soc. 2017 (139) 627.
(3) Li, G; Fürst, MJLJ; Mansouri, HR; Ressmann, AK; Ilie, A; Rudroff, F; Mihovilovic, MD; Fraaije, MW; Reetz, MT. Manipulating the Stereoselectivity of the Thermostable Baeyer-Villiger Monooxygenase TmCHMO by Directed Evolution. Org. Biomol. Chem.
2017 (15) 9824.
(4) Valencia, D; Guillén, M; Fürst, MJLJ; López-Santín, J; Álvaro, G. An Immobilized and Highly Stabilized Self-Sufficient Monooxygenase as Biocatalyst for Oxidative Biotransformations. J. Chem. Technol. Biotechnol. 2017 (93) 985.
(5) de Gonzalo, G; Fürst, MJLJ; Fraaije, MW. Polycyclic Ketone Monooxygenase (PockeMO): A Robust Biocatalyst for the Synthesis of Optically Active Sulfoxides.
Catalysts 2017 (7) 288.
(6) Delgove, MA; Fürst, MJLJ; Fraaije, MW; Bernaerts, KV; de Wildeman, SM. Exploring the Substrate Scope of Baeyer–Villiger Monooxygenases with Branched Lactones as Entry Towards Polyesters. ChemBioChem 2018 (19) 354.
(7) Fürst, MJLJ; Martin, C; Lončar, N; Fraaije, MW Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins. In:
Methods in Enzymology; Scrutton, NS (Ed.), Academic Press: 2018; Vol. 608; pp 151. (8) Wijma, HJ; Fürst, MJLJ; Janssen, DB A Computational Library Design Protocol for Rapid Improvement of Protein Stability: Fresco. In: Protein Engineering: Methods and Protocols; Bornscheuer, UT, Höhne, M (Eds.), Springer: New York, NY, 2018; Vol. 1685; pp 69-85.
(9) Li, G; Garcia-Borràs, M; Fürst, MJLJ; Ilie, A; Fraaije, MW; Houk, KN; Reetz, MT. Overriding Traditional Electronic Effects in Biocatalytic Baeyer-Villiger Reactions by Directed Evolution. J. Am. Chem. Soc. 2018 (140) 10464.
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(10) Fürst, MJLJ; Romero, E; Gómez Castellanos, JR; Fraaije, MW; Mattevi, A. Side-Chain Pruning Has Limited Impact on Substrate Preference in a Promiscuous Enzyme. ACS Catal. 2018 (8) 11648.
(11) Fürst, MJLJ; Fiorentini, F; Fraaije, MW; Beyond Active Site Residues: Overall Structural Dynamics Control Catalysis in Flavin-Containing and Heme-Containing Monooxygenases. Curr. Opin. Struct. Biol. 2019 (59) 29.
(12) Fürst, MJLJ; Kerschbaumer, B; Rinnofner, C; Winkler, M; Fraaije, MW; Exploring the Biocatalytic Potential of a Self-Sufficient Cytochrome P450 from
Thermothelomyces thermophila. Adv. Synth. Catal. 2019
(13) Volkwein, WV; Krafczyk, R; Jagtap, PKA; Parr, M; Mankina, E; Macošek, J; Guo, Z; Fürst, MJLJ; Pfab, M; Frishman, D; Hennig, J; Jung, K; Lassak, J; Switching the Post-Translational Modification of Translation Elongation Factor EF P. Front. Microbiol. 2019
(14) Fürst, MJLJ; Boonstra, M; Bandstra, S; Fraaije, MW; Stabilization of cyclohexanone monooxygenase by computational and experimental library design. Biotechnol. Bioeng. 2019
