Biochemical and molecular studies of atypical nevi
Nieuwpoort, A.F. van
Citation
Nieuwpoort, A. F. van. (2011, March 16). Biochemical and molecular studies of atypical nevi. Retrieved from https://hdl.handle.net/1887/16632
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Arno Frans van Nieuwpoort
Nieuwpoort, Arno Frans
Biochemical and molecular understanding of atypical nevi / Frans van Nieuwpoort.
Thesis Leiden.‐with ref.‐with summary in Dutch
Cover illustration: made by Joe DuBay. The cover illustrates cell culture of atypical melanocytes obtained from an atypical nevus.
All rights reserved. No part of this publication may be reproduced in any form without written consent of the copyright owner.
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© Arno Frans van Nieuwpoort, Leimuiden, 2011
Biochemical and molecular studies of atypical nevi
Proefschrift
ter verkrijging van
de graad van Doctor aan de Universiteit Leiden,
op gezag van de Rector Magnificus prof.mr. P.F.van der Heijden,
volgens besluit van het College voor Promoties
te verdedigen op woensdag 16 maart 2011
klokke 15:00 uur
door
Arno Frans van Nieuwpoort
geboren te Alphen aan den Rijn
in 1973
Promotiecommissie
Promotor: Prof. Dr. R. Willemze
Co‐promotor: Dr. N.A. Gruis
Overige leden: Prof. Dr. J. van den Oort
Prof. Dr. R.R. Frants
Prof. Dr. L.H.F. Mullenders
Voor Joe,
Voor mijn Ouders,
Chapter 1 Introduction
Chapter 2 Tyrosine‐Induced melanogenesis shows differences in morphologic and melanogenic preferences of melanosomes from light and dark Skin types
Chapter 3 Disturbed melanin synthesis and chronic oxidative stress in dysplastic nevi
Chapter 4 Increased melanogenesis is a risk factor for oxidative DNA damage‐ study on cultured melanocytes and atypical nevus cells
Chapter 5 Does oxidative stress drive melanoma development – new evidence from gene ontology studies on atypical nevi
Chapter 6 Proteomic analysis reveals reduced management of reactive oxygen species in atypical melanocytes
Chapter 7 Summary and Discussion
Nederlandse Samenvatting
Curriculum vitae
List of publications
List of abbreviations
α‐MSH Alpha‐melanocyt stimulating hormone
2D‐DIGE Two‐dimensional differential in‐gel electrophoresis AHP 4‐amino‐3‐hydroxyphenylalanine
AM Atypical melanocyte AN Atypical nevus AT 3‐amino‐L‐tyrosine
BRAF V‐raf murine sarcoma viral oncogene homolog B1 cAMP Cyclic adenosine monophosphate
CREB cAMP response element binding
CT Cholera toxin
DHR Dihydrorhodamine 123 DN Dysplastic nevi
DNA Deoxyribonucleic acid
FACS Fluorescence‐activated cell sorting
GO Gene onthology
GSH Glutathione
HPLC High‐performance liquid Chromatography IBMX Isobutylmethylxanthine
MAPK Mitogen‐activated protein (MAP) kinases
MC Melanocyte
MC1R Melanocortin 1 receptor
MITF Microphthalmia‐associated transcription factor
NM Normal melanocyte
NRAS Neuroblastoma RAS viral oncogene homolog PBS Phosphate buffered saline
PTCA Pyrrole‐2,3,5‐tricarboxylic acid ROS Reactive Oxygen Species SEM Standard error of the mean
TPA 12‐O‐Tetradecanoylphorbol‐13‐acetate UV Ultraviolet light
XRMA X‐ray microanalysis