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Biochemical and molecular studies of atypical nevi

Nieuwpoort, A.F. van

Citation

Nieuwpoort, A. F. van. (2011, March 16). Biochemical and molecular studies of atypical nevi. Retrieved from https://hdl.handle.net/1887/16632

Version: Corrected Publisher’s Version

License: Licence agreement concerning inclusion of doctoral thesis in the Institutional Repository of the University of Leiden

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Arno Frans van Nieuwpoort

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Nieuwpoort, Arno Frans   

Biochemical and molecular understanding of atypical nevi  / Frans van Nieuwpoort. 

 

Thesis Leiden.‐with ref.‐with summary in Dutch   

 

Cover illustration: made by Joe DuBay. The cover illustrates cell culture of atypical  melanocytes obtained from an atypical nevus. 

     

All rights reserved. No part of this publication may be reproduced in any form without  written consent of the copyright owner. 

 

Printed by Ipskamp    

© Arno Frans van Nieuwpoort, Leimuiden, 2011   

     

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Biochemical and molecular studies of atypical nevi

 

      Proefschrift 

 

ter verkrijging van   

de graad van Doctor aan de Universiteit Leiden,   

op gezag van de Rector Magnificus prof.mr. P.F.van der Heijden,   

volgens besluit van het College voor Promoties   

te verdedigen op woensdag 16 maart 2011   

klokke 15:00 uur   

door   

Arno Frans van Nieuwpoort   

geboren te Alphen aan den Rijn   

in 1973

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Promotiecommissie   

 

Promotor:     Prof. Dr. R. Willemze   

Co‐promotor:    Dr. N.A. Gruis   

Overige leden:   Prof. Dr. J. van den Oort 

      Prof. Dr. R.R. Frants 

      Prof. Dr. L.H.F. Mullenders   

                               

      Voor Joe, 

 

      Voor mijn Ouders, 

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Chapter 1  Introduction   

Chapter 2  Tyrosine‐Induced melanogenesis shows differences in morphologic and  melanogenic preferences of melanosomes from light and dark Skin types   

Chapter 3  Disturbed melanin synthesis and chronic oxidative stress in dysplastic nevi   

Chapter 4  Increased melanogenesis is a risk factor for oxidative DNA damage‐ study on  cultured melanocytes and atypical nevus cells 

 

Chapter 5  Does oxidative stress drive melanoma development – new       evidence from gene ontology studies on atypical nevi   

Chapter 6  Proteomic analysis reveals reduced management of reactive  oxygen species in atypical melanocytes 

 

Chapter 7  Summary and Discussion   

Nederlandse Samenvatting   

Curriculum vitae   

List of publications   

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List of abbreviations   

α‐MSH  Alpha‐melanocyt stimulating hormone 

2D‐DIGE  Two‐dimensional differential in‐gel electrophoresis  AHP  4‐amino‐3‐hydroxyphenylalanine 

AM  Atypical melanocyte  AN  Atypical nevus  AT  3‐amino‐L‐tyrosine 

BRAF  V‐raf murine sarcoma viral oncogene homolog B1  cAMP  Cyclic adenosine monophosphate 

CREB  cAMP response element binding 

CT  Cholera toxin 

DHR  Dihydrorhodamine 123  DN  Dysplastic nevi 

DNA  Deoxyribonucleic acid 

FACS  Fluorescence‐activated cell sorting 

GO  Gene onthology 

GSH  Glutathione 

HPLC  High‐performance liquid Chromatography  IBMX  Isobutylmethylxanthine 

MAPK  Mitogen‐activated protein (MAP) kinases 

MC  Melanocyte  

MC1R  Melanocortin 1 receptor 

MITF  Microphthalmia‐associated transcription factor 

NM  Normal melanocyte 

NRAS  Neuroblastoma RAS viral oncogene homolog  PBS  Phosphate buffered saline 

PTCA  Pyrrole‐2,3,5‐tricarboxylic acid  ROS  Reactive Oxygen Species  SEM  Standard error of the mean 

TPA  12‐O‐Tetradecanoylphorbol‐13‐acetate  UV  Ultraviolet light 

XRMA  X‐ray microanalysis   

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